A particulate enzyme preparation made from epicotyls of 1-week-old etiolated pea (Pisum sativum) seedlings was shown to incorporate glucuronic acid from UDP-D-[U-14C]glucuronic acid into a hemicellulosic polysaccharide. Optimum conditions for the incorporation include the presence of Mn2+ ions at between 4 and 10 mmol/litre and a pH between 5 and 6. UDP-D-xylose at 1 mmol/litre allows incorporation to continue for at least 8 h. In its absence, the reaction stops within 30 min. Analysis of the product by partial and total acid hydrolysis, followed by paper chromatography or electrophoresis, indicates that the polysaccharide produced is a glucuronoxylan.
Research Article| July 01 1983
A glucuronyltransferase involved in glucuronoxylan synthesis in pea (Pisum sativum) epicotyls
Biochem J (1983) 213 (1): 115–122.
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K W Waldron, C T Brett; A glucuronyltransferase involved in glucuronoxylan synthesis in pea (Pisum sativum) epicotyls. Biochem J 1 July 1983; 213 (1): 115–122. doi: https://doi.org/10.1042/bj2130115
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