A 1,4-beta-D-glucan cellobiohydrolase (EC 188.8.131.52) was purified from the culture liquid of Trichoderma reesei by using biospecific sorption on amorphous cellulose and immunoaffinity chromatography. A single protein band in polyacrylamide-gel electrophoresis and one arc in immunoelectrophoresis corresponded to the enzyme activity. The Mr was 65 000. The pI was 4.2-3.6. The purified enzyme contained about 10% hexose. The enzyme differs from previously described cellobiohydrolases in being more effective in the hydrolysis of cellulose.
Research Article|December 01 1983
Cellobiohydrolase from Trichoderma reesei
M L Niku-Paavola;
A Lappalainen; ;
Biochem J (1983) 215 (3): 677-683.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
M Nummi, M L Niku-Paavola, A Lappalainen, T M Enari, V Raunio; Cellobiohydrolase from Trichoderma reesei. Biochem J 1 December 1983; 215 (3): 677–683. doi: https://doi.org/10.1042/bj2150677
Download citation file: