Rat odontoblasts were shown to synthesize and secrete gamma-carboxyglutamic acid(Gla)-containing proteins into dentine after organ culture in the presence of radiolabelled amino acid precursors. Purified dentine Gla-containing protein from rat incisors was used as antigen to prepare rabbit antisera as a probe of dentine Gla-containing-protein biosynthesis in organ cultures of dentine (rat incisor) and bone (rat calvaria). Use of the antiserum also pointed out the cross-reactivity of a high-M, glycoprotein present within the dentine matrix. The present results are significant in identifying dentine gla-containing protein as endogenous to mineralizing dentine and may relate to the commonality between calcifying connective tissues in general.
The biosynthesis of dentine γ-carboxyglutamic acid-containing proteins by rat incisor odontoblasts in organ culture
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M T DiMuzio, M Bhown, W T Butler; The biosynthesis of dentine γ-carboxyglutamic acid-containing proteins by rat incisor odontoblasts in organ culture. Biochem J 15 November 1983; 216 (2): 249–257. doi: https://doi.org/10.1042/bj2160249
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