Inhibitors of lysosomal acidification (4,4′-di-isothiocyanostilbene-2,2′-disulphonate, NN'-dicyclohexylcarbodi-imide, carbonyl cyanide m-chlorophenylhydrazone, NH4Cl and methylamine hydrochloride) did not alter cystine egress or countertransport in polymorphonuclear-leucocyte lysosome-rich granular fractions at pH 7.0. Together, 2 mM-MgCl2/MgATP and 90 mM-KCl stimulated cystine egress 2-fold, but this effect also was not influenced by inhibitors of ATP-dependent lysosomal acidification. MgCl2/MgATP stimulated cystine transport at pH 5.5, but the effect also occurred with MgCl2, MgSO4 or MnCl2 alone, was prevented by chelation, and was not seen with NaATP; therefore, it was considered a bivalent-cation, not an ATP, effect. Proton-pump-mediated acidification of lysosomes does not appear to be required for cystine transport in normal polymorphonuclear-leucocyte granular fractions, as reported for lymphoblast lysosomes.
Research Article|May 15 1985
pH effects on cystine transport in lysosome-rich leucocyte granular fractions
Biochem J (1985) 228 (1): 263-267.
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W A Gahl, F Tietze; pH effects on cystine transport in lysosome-rich leucocyte granular fractions. Biochem J 15 May 1985; 228 (1): 263–267. doi: https://doi.org/10.1042/bj2280263
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