Since the exposure of rats to cadmium causes zinc to accumulate in metallothionein in liver and kidney but not in a similar protein in the testes, the properties of the low-Mr cadmium-binding proteins were investigated in rat testes. Weanling rats that had been given dietary cadmium for 6 weeks were injected with 109CdCl2 and subsequently killed, and the 109Cd-labelled low-Mr proteins from testes were purified. The pooled low-Mr cadmium-containing fractions from the gel-filtration (Sephadex G-75) columns were eluted through DEAE-Sephacel columns, yielding two peaks. Each of the individual peaks from this Sephacel column was further purified by rechromatography on DEAE-Sephacel and on Bio-Gel P-10 columns. Amino acid analysis of the two purified proteins revealed a low cysteine (about 3%) content, with aspartate, glutamate and glycine as the predominant amino acids. Thus these low-Mr cadmium-binding proteins induced by cadmium in rat testes do not appear to be metallothionein.
Research Article|October 15 1985
Properties of cadmium-binding proteins in rat testes. Characteristics unlike metallothionein
Biochem J (1985) 231 (2): 279-283.
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J T Deagen, P D Whanger; Properties of cadmium-binding proteins in rat testes. Characteristics unlike metallothionein. Biochem J 15 October 1985; 231 (2): 279–283. doi: https://doi.org/10.1042/bj2310279
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