N-Bromosuccinimide cleavage of a lysine-rich histone fraction (histone III-S) yields a peptide substrate, purified by reverse-phase h.p.l.c., for the Ca2+ + phospholipid-dependent protein kinase (protein kinase C). This substrate displays no reactivity with the cyclic AMP-dependent protein kinase, and may prove useful for the detection of protein kinase C activity in crude tissue extracts.
Research Article|October 15 1985
Assay of protein kinase C with an N-bromosuccinimide-cleavage fragment of histone H1
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Biochem J (1985) 231 (2): 489-492.
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B Glynn, J Colliton, J McDermott, L A Witters; Assay of protein kinase C with an N-bromosuccinimide-cleavage fragment of histone H1. Biochem J 15 October 1985; 231 (2): 489–492. doi: https://doi.org/10.1042/bj2310489
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