A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an Mr of 100000 was observed. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.
Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
G C Ness, C E Sample, M Smith, L C Pendleton, D C Eichler; Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Biochem J 1 January 1986; 233 (1): 167–172. doi: https://doi.org/10.1042/bj2330167
Download citation file: