The sugar nucleotide analogue UDP-glucosamine was found to function as a sugar donor in microsomal preparations of both chick-embryo cells and rat liver, yielding dolichyl monophosphate glucosamine (Dol-P-GlcN). This was characterized by t.l.c. and retention by DEAE-cellulose. Glucosamine was the only water-soluble product released on mild acid hydrolysis. Dol-P-GlcN did not serve as substrate by transferring its glucosamine moiety to dolichol-linked oligosaccharide. Competition experiments between UDP-[3H]glucose and UDP-glucosamine showed Dol-P-[3H]glucose synthesis to be depressed by 56 or 73% in microsomes from chick-embryo cells and rat liver respectively. The concentrations of the UDP-sugars in this experiment were comparable with those occurring in galactosamine-metabolizing liver. These findings suggest that Dol-P-GlcN, formed as a metabolite of D-galactosamine, may interfere with Dol-P-dependent reactions.
Research Article|February 01 1986
UDP-glucosamine as a substrate for dolichyl monophosphate glucosamine synthesis
D O R Keppler;
Biochem J (1986) 233 (3): 749-754.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
W McDowell, G Weckbecker, D O R Keppler, R T Schwarz; UDP-glucosamine as a substrate for dolichyl monophosphate glucosamine synthesis. Biochem J 1 February 1986; 233 (3): 749–754. doi: https://doi.org/10.1042/bj2330749
Download citation file: