The binding to purified calmodulin of five spin-labelled derivatives of chlorophenothiazine was investigated by e.s.r. spectrometry and by the antagonizing potency on the calmodulin-dependent activation of myosin light chain kinase. The results of a comparative study and the influence of pH and ionic strength on the binding support the occurrence of an electrostatic binding involving the terminal amino group of the side-chain of the chlorophenothiazine. These results are discussed in relation to the specificity of the interaction that holds the antipsychotic drug-calmodulin complex together.
Research Article|February 01 1986
Interaction between calmodulin and five different spin-labelled chlorophenothiazines
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Biochem J (1986) 233 (3): 853-857.
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J L Olivier, D Rainteau, G Bereziat, C Wolf; Interaction between calmodulin and five different spin-labelled chlorophenothiazines. Biochem J 1 February 1986; 233 (3): 853–857. doi: https://doi.org/10.1042/bj2330853
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