Two homologous series of urinary oligosaccharides were identified by h.p.l.c. and fast-atom-bombardment mass spectrometry in feline alpha-mannosidosis. The predominant series has the composition Man2-8GlcNAc2 and a minor series the composition Man2-9GlcNAc. The structure of the most abundant oligosaccharide, which accounts for over 80% of the urinary oligosaccharide, was shown to be alpha-D-Manp(1—3)[alpha-D-Manp-(1—6)]beta-D-Manp -(1—4)-beta-D-GlcpNA c-(1—4)-D-GlcNAc by gas chromatography and mass spectrometry. Such a structure is consistent with the incomplete catabolism of complex N-linked glycans due to a deficiency of alpha-D-mannosidase in tissue lacking an endohexosaminidase activity.
Structural analysis of the major urinary oligosaccharides in feline α-mannosidosis
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D Abraham, P Daniel, A Dell, J Oates, R Sidebotham, B Winchester; Structural analysis of the major urinary oligosaccharides in feline α-mannosidosis. Biochem J 1 February 1986; 233 (3): 899–904. doi: https://doi.org/10.1042/bj2330899
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