The affinities for hyaluronic acid of newly synthesized proteoglycan from post-confluent rabbit chondrocyte cultures and purified bovine proteoglycan monomer were compared. In mixtures prepared at pH 6.8 the newly synthesized proteoglycan had the lower affinity; however, in mixtures incubated at pH 8.5 for 24 h before addition of hyaluronic acid, the newly synthesized proteoglycan exhibited a markedly higher affinity than the bovine monomer. The results suggest that proteoglycan secreted without associated link protein [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] has a low affinity for hyaluronate and that this may be increased during subsequent extracellular processing.
Research Article|February 15 1986
The affinity of newly synthesized proteoglycan for hyaluronic acid can be enhanced by exposure to mild alkali
Biochem J (1986) 234 (1): 221-223.
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A H K Plaas, J D Sandy; The affinity of newly synthesized proteoglycan for hyaluronic acid can be enhanced by exposure to mild alkali. Biochem J 15 February 1986; 234 (1): 221–223. doi: https://doi.org/10.1042/bj2340221
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