The present communication describes a novel method for estimating initial velocities (v) of enzyme-catalysed reactions. It is based on an approximation of experimental data obtained by the cubic spline function. The initial velocity of a reaction is calculated as a derivative of the approximating function at a time value equal to zero. The proposed method is usable on a computer with a FORTRAN IV program. The method can be successfully used in such cases as substantial extents of substrate conversion, the inactivation of an enzyme in the course of a reaction, the existence of large experimental error or when the reaction mechanism is unknown.
Research Article|August 01 1986
Determination of initial velocities of enzymic reactions from progress curves
A Pauliukonis; ;
Biochem J (1986) 237 (3): 821-825.
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R Dagys, A Pauliukonis, D Kazlauskas, M Mankevicius, R Simutis; Determination of initial velocities of enzymic reactions from progress curves. Biochem J 1 August 1986; 237 (3): 821–825. doi: https://doi.org/10.1042/bj2370821
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