The germination response of spores of Bacillus megaterium KM, as measured by loss of A600, is more than 95% inhibited by 1 mM-HgCl2. Two Hg2+-sensitive sites (referred to as ‘sites I and II’) have been identified during germination. Site I represents a pre-commitment event and can be protected from HgCl2 by 50 mM-D-alanine, whereas site II represents a post-commitment event and is not D-alanine-protectable. At 1 mM-HgCl2, 25% of the spore population becomes committed to germinate, but an A600 loss of less than 5% occurs. In this system, loss of heat resistance was associated with commitment, whereas selective cortex hydrolysis, release of pyridine-2,6-dicarboxylic acid, Zn2+ and soluble peptidoglycan, as well as loss of refractility, were identified as post-commitment events. The commitment event was reversibly inhibited by several proteinase inhibitors and a membrane bulking agent. A model of spore germination based on these results is presented.
Research Article|August 01 1986
The use of inhibitors to identify early events during Bacillus megaterium KM spore germination
Biochem J (1986) 237 (3): 865-870.
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S J Foster, K Johnstone; The use of inhibitors to identify early events during Bacillus megaterium KM spore germination. Biochem J 1 August 1986; 237 (3): 865–870. doi: https://doi.org/10.1042/bj2370865
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