The existence of a GTP-binding protein of the Ns type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent Mr approx. 45,000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the Ns protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the Ns protein provided by T. cruzi epimastigotes.
Evidence for the existence of an Ns-type regulatory protein in Trypanosoma cruzi membranes
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C D Eisenschlos, A A Paladini, L Molina y Vedia, H N Torres, M M Flawiá; Evidence for the existence of an Ns-type regulatory protein in Trypanosoma cruzi membranes. Biochem J 1 August 1986; 237 (3): 913–917. doi: https://doi.org/10.1042/bj2370913
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