Monoclonal antibodies have been raised against rat kidney gamma-glutamyl transpeptidase (GGT). All five antibodies immunoprecipitate enzyme activity from solubilized kidney brush-border membranes, but not from hepatocellular carcinoma membranes. Three of the antibodies react immunohistochemically with brush-border membranes in sections of adult rat kidney, but none of the antibodies cross-react with sections of guinea-pig, mouse or marmoset kidney or with untreated or carcinogen-treated rat liver. The antibodies do not recognize GGT in foetal-rat kidney and react poorly with kidney from 2-year-old rat. They do react with tubules trapped within mesenchymal kidney tumours induced by dimethylnitrosamine, but epithelial tumours, which are GGT-positive (although much less so than normal kidney), are not immunoreactive.
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September 1986
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Research Article|
September 15 1986
Monoclonal antibodies against rat kidney γ-glutamyl transpeptidase show species and tissue specificity
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1986 London: The Biochemical Society
1986
Biochem J (1986) 238 (3): 913–917.
Citation
J A Green, N D Cook, M M Manson; Monoclonal antibodies against rat kidney γ-glutamyl transpeptidase show species and tissue specificity. Biochem J 15 September 1986; 238 (3): 913–917. doi: https://doi.org/10.1042/bj2380913
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