The methanogenic bacterium Methanobacterium thermoautotrophicum (A.T.C.C. 29183) was shown to contain two new aminophospholipids. These are 2-aminoethyl phosphate ester of diphytanylglycerol diether and a sugar containing bisdiphytanyldiglycerol tetraether. The two aminophospholipids were stable to acid methanolysis except for the sugar on the bisdiphytanyldiglycerol tetraether. Strong acid (6 M-HCl) hydrolysed the alkyl ether and aminophosphate ester bonds. The structure of the phosphate linkage was demonstrated by 31P n.m.r., and the 2-ethanolamine structure was elucidated by 1H- and 13C-n.m.r. spectroscopy and by fast-atom-bombardment m.s.
Research Article|July 01 1987
Structure of two new aminophospholipids from Methanobacterium thermoautotrophicum
J K G Kramer;
F D Sauer;
Biochem J (1987) 245 (1): 139-143.
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J K G Kramer, F D Sauer, B A Blackwell; Structure of two new aminophospholipids from Methanobacterium thermoautotrophicum. Biochem J 1 July 1987; 245 (1): 139–143. doi: https://doi.org/10.1042/bj2450139
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