Exposure of murine L929 fibroblasts to ozone caused inactivation of the energy-coupled transport systems for 2-aminoisobutyric acid and Rb+. Ozone induced an increase of the apparent Km for 2-aminoisobutyric acid transport, with a constant Vmax, indicating impairment of the carrier function, rather than a decrease in the number of transport sites. The uptake rate of 2-deoxy-D-glucose, on the other hand, increased after exposure of the cells to ozone. This was caused by an increased Vmax. of facilitated diffusion, without increased after ozone exposure. Together with the K+ leakage described previously, this reflects ozone-induced impairment of the barrier function of the membrane.
Toxic effects of ozone on murine L929 fibroblasts. Damaging action on transmembrane transport systems
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
J Van der Zee, T M A R Dubbelman, J Van Steveninck; Toxic effects of ozone on murine L929 fibroblasts. Damaging action on transmembrane transport systems. Biochem J 1 July 1987; 245 (1): 301–304. doi: https://doi.org/10.1042/bj2450301
Download citation file: