A technique is described using ACCELL QMA anion-exchange SEP-PAKs (Waters Associates) with ammonium formate-based solutions, whereby a sample can be processed within minutes to yield resolution of inositol phosphates. Isomers of inositol trisphosphate can then be separated by using this technique in combination with a rapid (5-6 min) isocratic h.p.l.c. procedure. The use of QMA SEP-PAKs offers a degree of reproducibility comparable with that of h.p.l.c. while maintaining the capacity for automation, allowing large numbers of samples to be processed rapidly.
Research Article|August 01 1987
A rapid separation method for inositol phosphates and their isomers
K A Wreggett;
Biochem J (1987) 245 (3): 655-660.
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K A Wreggett, R F Irvine; A rapid separation method for inositol phosphates and their isomers. Biochem J 1 August 1987; 245 (3): 655–660. doi: https://doi.org/10.1042/bj2450655
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