Pseudomonas toxin was found to bind Triton X-114 at pH values below pH 5.0, whereas much less binding was observed at higher pH values. The toxin bound Triton X-114 at a higher pH value in the presence of 0.14 M-NaCl, -KCl or -NaNO3 than at low salt concentrations. The results suggest that low pH in an intracellular compartment facilitates the transport of Pseudomonas toxin across the membrane and into the cytosol by inducing a conformational change in the molecule.

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