A conflict exists in the literature concerning the mode of translocation of D-glucose and D-ribose across the lysosome membrane. The more rapid net uptake of ribose, when measured by the osmotic-protection technique, has been attributed either to its smaller size and lower hydrogen-bonding capacity, or to a lower affinity for a transport system shared by both sugars. The latency of acid beta-hexosaminidase in isolated rat liver lysosomes was measured after preincubation for periods up to 1 h in various solutions containing glucose and/or ribose, and in some cases sucrose. After confirmation of the superior osmotic protection afforded by glucose (than by ribose), it was shown that a solution 0.125 M in both glucose and ribose provided protection intermediate between that given by 0.25 M-glucose and that given by 0.25 M-ribose. This result is inconsistent with the common-carrier hypothesis.
Research Article|August 01 1987
Translocation of sugars into rat liver lysosomes. Evidence against a common carrier for d-glucose and d-ribose
S J Bird;
Biochem J (1987) 245 (3): 929-931.
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S J Bird, S Forster, J B Lloyd; Translocation of sugars into rat liver lysosomes. Evidence against a common carrier for d-glucose and d-ribose. Biochem J 1 August 1987; 245 (3): 929–931. doi: https://doi.org/10.1042/bj2450929
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