The transport of glycine in C6 glioma cells takes place mainly in a heterogeneous Na+-dependent manner which can be resolved into different components. A Na+- and Cl(-)-dependent component with high affinity for glycine is pH-sensitive and inhibited by sarcosine, all these characteristics corresponding to System Gly. The low-affinity component of the transport of glycine can be discriminated as two components, namely System A and System ASC. The main proportion of glycine transport through the low-affinity system is carried out by the ASC System, which appears to be constitutively expressed by the cells. The adaptive response of the low-affinity Na+-dependent transport of glycine to amino acid deprivation was identified with System A on the basis of its ion-dependency, pH-sensitivity and by inhibition analysis. The possible physiological role of the high- and low-affinity components of the transport system for glycine in glial cells is discussed.
Research Article| March 01 1989
Characteristics and adaptive regulation of glycine transport in cultured glial cells
Biochem J (1989) 258 (2): 403–408.
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F Zafra, C Giménez; Characteristics and adaptive regulation of glycine transport in cultured glial cells. Biochem J 1 March 1989; 258 (2): 403–408. doi: https://doi.org/10.1042/bj2580403
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