When the production of bilirubin by biliverdin reductase was monitored at 460 nm by stopped-flow spectrophotometry a ‘burst’ was observed with a first-order rate constant at pH 8 of 20 s-1. The steady-state rate was established on completion of the ‘burst’. When the reaction was monitored at 401 nm there was no observed steady-state rate, but a diminished pre-steady-state ‘burst’ reaction was still seen with a rate constant of 22 s-1. We argue that the rate-limiting reaction is the dissociation of bilirubin from an enzyme.NADP+.bilirubin complex. With NADPH as the cofactor the hydride-transfer step was shown to exhibit pH-dependence associated with an ionizing group with a pK of 7.2. The kinetics of NADPH binding to the enzyme at pH 7.0 were measured by monitoring the quenching of protein fluorescence on binding the coenzyme.
The kinetics of ox kidney biliverdin reductase in the pre-steady state. Evidence that the dissociation of bilirubin is the rate-determining step
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E Rigney, T J Mantle, F M Dickinson; The kinetics of ox kidney biliverdin reductase in the pre-steady state. Evidence that the dissociation of bilirubin is the rate-determining step. Biochem J 1 May 1989; 259 (3): 709–713. doi: https://doi.org/10.1042/bj2590709
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