Ouabain binding was studied in Xenopus laevis oocytes permeabilized by detergents. The behaviour of markers showed that 10 microM-digitonin selectively disrupts the plasma membrane. In the presence of ATP, oocytes permeabilized at 10 microM-digitonin bound no more ouabain molecules than were required to abolish active 86Rb+ uptake in the intact cells. However, the ouabain binding capacity increased approx. 2-fold when inner membranes were disrupted by SDS or excess digitonin, as judged from the accompanying release of the lysosomal marker beta-hexosaminidase. The results suggest that oocytes have a large internal pool of functional sodium pumps.
Research Article|June 01 1989
Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes
Biochem J (1989) 260 (2): 395-399.
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G Schmalzing, S Kröner, H Passow; Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes. Biochem J 1 June 1989; 260 (2): 395–399. doi: https://doi.org/10.1042/bj2600395
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