Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.
Research Article|June 01 1989
The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation
Biochem J (1989) 260 (2): 593-596.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
S Palmer, M J O Wakelam; The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation. Biochem J 1 June 1989; 260 (2): 593–596. doi: https://doi.org/10.1042/bj2600593
Download citation file: