GSSG selectively elutes two GSH transferases from a mixture of rat GSH transferases bound to a GSH-agarose affinity matrix. One is a form of GSH transferase 1-1 and the other is shown to be GSH transferase 8-8. By using tissues that lack this form of GSH transferase 1-1 (e.g. lung), GSH transferase 8-8 may thus be purified from cytosol in a single step. Quantitative analysis of the tissue distribution of GSH transferase 8-8 was obtained by h.p.l.c.
Single-step purification and h.p.l.c. analysis of glutathione transferase 8–8 in rat tissues
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D J Meyer, E Lalor, B Coles, A Kispert, P Ålin, B Mannervik, B Ketterer; Single-step purification and h.p.l.c. analysis of glutathione transferase 8–8 in rat tissues. Biochem J 15 June 1989; 260 (3): 785–788. doi: https://doi.org/10.1042/bj2600785
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