In the cloned rat thymic endocrine epithelial cell line TEA3A1, treatment with dexamethasone leads to decreased levels of prostaglandin E2, prostaglandin F2 alpha, and thromboxane B2. Dexamethasone treatment also leads to a decrease of both calcium-dependent and calcium-independent phospholipase A2 activity measured in a cell-free assay. Dexamethasone-treated cells also have increased levels of lipocortin-I, a putative modulator of phospholipase A2 activity. The property of calcium-dependent binding of lipocortin to the particulate fraction was used to prepare cytosolic and particulate subcellular fractions which contained phospholiphase A2 activity but no lipocortin-I. Dexamethasone decreased phospholipase A2 activity in both cytosolic and particulate fractions even in the absence of lipocortin, suggesting the presence of a lipocortin-independent mechanism.
Research Article|July 15 1989
Lipocortin-independent effect of dexamethasone on phospholipase activity in a thymic epithelial cell line
R A Fava;
Biochem J (1989) 261 (2): 395-400.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
A Piltch, L Sun, R A Fava, J Hayashi; Lipocortin-independent effect of dexamethasone on phospholipase activity in a thymic epithelial cell line. Biochem J 15 July 1989; 261 (2): 395–400. doi: https://doi.org/10.1042/bj2610395
Download citation file: