The vasoactive peptide endothelin is shown to be a potent mitogen for Swiss 3T3 cells. Although endothelin has little effect on DNA synthesis when added alone to cells in serum-free medium, the peptide synergizes very strongly with several other growth factors. A half-maximal response to endothelin is observed at approx. 0.3 nM, with a maximal effect at 3 nM. Over the same concentration range, endothelin stimulates a 2-fold increase in the accumulation of cellular inositol phosphates. Endothelin may prove to be a useful additional agonist for studying the signalling pathways involved in the control of 3T3-cell proliferation.
Research Article|November 01 1989
Endothelin stimulates DNA synthesis in Swiss 3T3 cells. Synergy with polypeptide growth factors
K D Brown;
Biochem J (1989) 263 (3): 977-980.
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K D Brown, C J Littlewood; Endothelin stimulates DNA synthesis in Swiss 3T3 cells. Synergy with polypeptide growth factors. Biochem J 1 November 1989; 263 (3): 977–980. doi: https://doi.org/10.1042/bj2630977
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