Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane protein (TGN38) which is located specifically in the trans-Golgi network.
Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38)
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J P Luzio, B Brake, G Banting, K E Howell, P Braghetta, K K Stanley; Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38). Biochem J 15 August 1990; 270 (1): 97–102. doi: https://doi.org/10.1042/bj2700097
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