The secretion of a glutathione-S-conjugate, dinitrophenyl-glutathione (GS-DNP) was studied in the Caco-2 cells, a cultured human colonic adenocarcinoma cell line with many of the characteristics of enterocytes. The labelled glutathione conjugate was generated within the cell by incubation with 14C-labelled 1-chloro-2,4-dinitrobenzene (CDNB). This compound is hydrophobic and enters the cell by simple diffusion. Cells incubated with CDNB at 10 degrees C form only one metabolite, GS-DNP. After secretion into the medium GS-DNP is partly converted into one or two slightly more hydrophobic products. This must represent hydrolysis of the glutathione moiety by the action of gamma-glutamyltransferase (EC 184.108.40.206.; gamma-GT) because the reaction was completely inhibited by acivicin, an inhibitor of gamma-GT. Secretion of GS-DNP was a temperature-sensitive, saturable process with an apparent Km of 1.03 +/- 0.26 nmol/mg of protein and a Vmax of 111 +/- 17 pmol/min per mg of protein. The secretion was not sensitive to trans-stimulation by extracellular concentrations of GS-DNP up to 2.5 mM. Furthermore the initial GS-DNP secretion rate was sensitive to dissipation of the membrane potential and correlated closely with the cellular ATP content. Caco-2 cells cultured on nitrocellulose filters secreted GS-DNP significantly faster over the basolateral membrane than over the apical membrane (146 +/- 25 versus 90 +/- 18 pmol/min per mg respectively). Secretion over both membrane domains of the cell was sensitive to ATP depletion. In conclusion, Caco-2 cells contain an active-transport system that is primarily involved in the secretion of glutathione conjugates and that is present in both plasma membrane domains of the cell.
Research Article|March 15 1993
Glutathione-conjugate transport by human colon adenocarcinoma cells (Caco-2 cells)
R P J Oude Elferink;
C T M Bakker;
Biochem J (1993) 290 (3): 759-764.
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R P J Oude Elferink, C T M Bakker, P L M Jansen; Glutathione-conjugate transport by human colon adenocarcinoma cells (Caco-2 cells). Biochem J 15 March 1993; 290 (3): 759–764. doi: https://doi.org/10.1042/bj2900759
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