Two monoclonal antibodies against the 17 beta-oestradiol dehydrogenase of pig endometrial cells have been used in localization studies with immunogold electron microscopy. The antibodies attach both to a fraction of dehydrogenase-rich cytoplasmic vesicles isolated from homogenates and to vesicles of similar appearance in cells. The vesicles are filled with electron-dense material. Their tagging intensity indicates a high degree of specialization. Endometrial cells from mature animals contain a host of dehydrogenase vesicles, and cells from prepubertal animals only a few. Functional aspects of the novel organelle are discussed.
Research Article|March 15 1993
The 17β-oestradiol dehydrogenase of pig endometrial cells is localized in specialized vesicles
Biochem J (1993) 290 (3): 777-782.
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J Adamski, B Husen, F Marks, P W Jungblut; The 17β-oestradiol dehydrogenase of pig endometrial cells is localized in specialized vesicles. Biochem J 15 March 1993; 290 (3): 777–782. doi: https://doi.org/10.1042/bj2900777
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