The synthesis of 2-carboxy-D-arabinitol-1-phosphate (CA1P), the naturally occurring inhibitor of ribulose-1,5-bisphosphate carboxylase/oxygenase, was studied in leaves of the French bean Phaseolus vulgaris, L. Leaves were supplied with air containing 14CO2 in the light then the plants were transferred to normal air in the light or in the dark. Leaf samples were frozen in liquid nitrogen, ground to a powder and extracted with acid. Lipids, pigments and cations were removed from the extract and CA1P and 2-carboxy-D-arabinitol (CA) recovered by anion exchange chromatography. The CA1P was further purified by its specific binding to purified ribulose-1,5-bisphosphate carboxylase/oxygenase. CA and CA1P were identified by chromatographic properties and n.m.r. spectra. When plants were kept for 15 h in darkness after exposure to 14CO2, up to 2.2% and 5.5% of the radioactivity in the extracts was present in CA1P and CA, respectively. The most radioactivity appeared in these compounds when photosynthesis from 14CO2 took place at low photosynthetic photon flux density (PPFD). Under such conditions, radioactivity was detected in CA1P after only 10 min. During subsequent exposure to normal air (12CO2) at low PPFD the amount of radioactivity in CA1P remained almost constant for 6 h; in darkness the rate of incorporation of radioactivity into CA1P reached a maximum after 2 h and the radioactivity was still increasing 6 h later. At low PPFD, the amount of CA1P in the leaves reached a maximum after 2 h. In darkness, the amount of CA1P began to increase rapidly after a lag of almost 1 h, well ahead of the increase in radioactivity in CA1P.
Skip Nav Destination
Article navigation
December 1994
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
December 15 1994
Incorporation of carbon from photosynthetic products into 2-carboxyarabinitol-1-phosphate and 2-carboxyarabinitol
P J Andralojc;
P J Andralojc
1Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire, AL5 2JQ, U.K.
Search for other works by this author on:
G W Dawson;
G W Dawson
1Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire, AL5 2JQ, U.K.
Search for other works by this author on:
M A J Parry;
M A J Parry
1Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire, AL5 2JQ, U.K.
Search for other works by this author on:
A J Keys
A J Keys
1Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire, AL5 2JQ, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1994 The Biochemical Society, London
1994
Biochem J (1994) 304 (3): 781–786.
Citation
P J Andralojc, G W Dawson, M A J Parry, A J Keys; Incorporation of carbon from photosynthetic products into 2-carboxyarabinitol-1-phosphate and 2-carboxyarabinitol. Biochem J 15 December 1994; 304 (3): 781–786. doi: https://doi.org/10.1042/bj3040781
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.