The action of non-detergent sulphobetaines (NDSBs) as new mild agents for protein purification is described. The solubilization effects of non-detergent sulphobetaines are shown in different examples; all obtained under non-denaturing conditions: (1) microsomal proteins extraction; (2) recovery after dialysis of nuclear proteins; (3) reduction of precipitation in isoelectric focusing experiments under non-denaturing conditions; and (4) purification of a membrane-bound serine protease from Plasmodium falciparum involved in erythrocyte invasion by malaria merozoites. The absence of a significant denaturation effect induced by NDSBs is demonstrated by tests on beta-galactosidase and alkaline phosphatase. A simple NDSB synthesis and some possible explanations of the action of NDSBs are also presented.
Research Article| January 01 1995
Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Biochem J (1995) 305 (1): 337–343.
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L Vuillard, C Braun-Breton, T Rabilloud; Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification. Biochem J 1 January 1995; 305 (1): 337–343. doi: https://doi.org/10.1042/bj3050337
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