The expression of cell-surface haemopexin (Hx) receptors on human cytotrophoblasts was assessed by using four different Hx species purified from plasma: human Hx isolated by wheatgerm-affinity chromatography, human Hx isolated by haem-agarose-affinity chromatography and rabbit and rat Hx, also isolated by haem-agarose-affinity chromatography. About 3500-7000 high-affinity (Kd 0.34-0.85 nM) receptors per cell were measured by Scatchard-type analysis at 4 degrees C using human (species obtained by both methods) or rabbit 125I-labelled haem-Hx. Measured simultaneously, transferrin receptor number and affinity were 40,000/cell and 0.83 nM respectively. In contrast with transferrin receptors, the number of Hx receptors did not increase during 24 h in cytotrophoblast culture. Rat Hx showed no specific binding to human Hx receptors in cytotrophoblast cultures.
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May 1995
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Research Article|
May 01 1995
Expression of haemopexin receptors by cultured human cytotrophoblast
H P van Dijk;
H P van Dijk
*Department of Chemical Pathology, Erasmus Universiteit Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
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M J Kroos;
M J Kroos
*Department of Chemical Pathology, Erasmus Universiteit Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
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J S Starreveld;
J S Starreveld
*Department of Chemical Pathology, Erasmus Universiteit Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
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H G van Eijk;
H G van Eijk
*Department of Chemical Pathology, Erasmus Universiteit Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
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S P Tang;
S P Tang
†Deparment of Pediatrics, Biochemistry and Pharmacology, Cornell University Medical College, New York, NY 10021, U.S.A.
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D X Song;
D X Song
†Deparment of Pediatrics, Biochemistry and Pharmacology, Cornell University Medical College, New York, NY 10021, U.S.A.
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U Muller-Eberhard
U Muller-Eberhard
†Deparment of Pediatrics, Biochemistry and Pharmacology, Cornell University Medical College, New York, NY 10021, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1995 The Biochemical Society, London
1995
Biochem J (1995) 307 (3): 669–672.
Citation
H P van Dijk, M J Kroos, J S Starreveld, H G van Eijk, S P Tang, D X Song, U Muller-Eberhard; Expression of haemopexin receptors by cultured human cytotrophoblast. Biochem J 1 May 1995; 307 (3): 669–672. doi: https://doi.org/10.1042/bj3070669
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