A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.
Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D
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Krishna M. ELLA, Joseph W. DOLAN, Chen QI, Kathryn E. MEIER; Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D. Biochem J 15 February 1996; 314 (1): 15–19. doi: https://doi.org/10.1042/bj3140015
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