Pili, which are filamentous protein structures on the surface of the meningitis-causing organism Neisseria meningitidis, are known to be post-translationally modified with substituents that affect their mobility in SDS/PAGE and which might play a crucial role in adherence and bloodstream invasion. Tryptic digests of pili were analysed by fast atom bombardment and electrospray MS to identify putative modifications. Serine-93 was found to carry a novel modification of α-glycerophosphate. This is the first time that α-glycerophosphate has been observed as a substituent of a prokaryotic or eukaryotic protein.
Discovery of a novel protein modification: α-glycerophosphate is a substituent of meningococcal pilin
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Elaine STIMSON, Mumtaz VIRJI, Stephanie BARKER, Maria PANICO, Ian BLENCH, Jon SAUNDERS, Gail PAYNE, E. Richard MOXON, Anne DELL, Howard R. MORRIS; Discovery of a novel protein modification: α-glycerophosphate is a substituent of meningococcal pilin. Biochem J 15 May 1996; 316 (1): 29–33. doi: https://doi.org/10.1042/bj3160029
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