Substrate isotope studies, solvent isotope studies, proton in-ventories and studies of Vmax and Km as a function of pH suggest an E1CB (V. E. Anderson (1991) in Enzyme Mechanisms for Isotope Effects (Cook, P. F., ed.), pp. 389–417, CRC Press, Boca Raton, FL) mechanism via an enolate intermediate for type II dehydroquinases involved in biosynthetic or catabolic pathways.
Evidence from kinetic isotope studies for an enolate intermediate in the mechanism of type II dehydroquinases
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
Joanna M HARRIS, Concepcion GONZALEZ-BELLO, Colin KLEANTHOUS, Alastair R. HAWKINS, John R. COGGINS, Chris ABELL; Evidence from kinetic isotope studies for an enolate intermediate in the mechanism of type II dehydroquinases. Biochem J 15 October 1996; 319 (2): 333–336. doi: https://doi.org/10.1042/bj3190333
Download citation file: