The effects of the rotational information of DNA in determining the in vivolocalization of nucleosomal core particles (ncps) have been studied in the Saccharomyces cerevisiae5 S rRNA repeat gene. The distribution of the phased series of flexibility signals present in this DNA has been altered by inserting in its centre a 25 bp tract. The effects of such alteration on the in vivodistribution of the helically phased, alternatively located ncps have been determined relative to a reference 21 bp insertion mutant. The results show that the answers provided in vitroand in vivoby the yeast 5 S rRNA gene sequence to specific modifications of the DNA rotational frame are similar, thus pointing to the relevance of DNA rotational information in vivo.
Changing nucleosome positions in vivo through modification of the DNA rotational information
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Lucia DI MARCOTULLIO, Memmo BUTTINELLI, Giovanna COSTANZO, Ernesto DI MAURO, Rodolfo NEGRI; Changing nucleosome positions in vivo through modification of the DNA rotational information. Biochem J 1 July 1998; 333 (1): 65–69. doi: https://doi.org/10.1042/bj3330065
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