Rheumatoid arthritis and periodontitis are inflammatory diseases modulated by proinflammatory cytokines [e.g. interleukin (IL-1) 1 and tumour necrosis factor α], which activate local fibroblasts to do the following: (1) proliferate, (2) induce gene expression and (3) produce destructive metalloproteinases. Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor (composed of HIF-1α and HIF-1β/aryl hydrocarbon receptor nuclear transporter) that is modulated by hypoxia. HIF-1 binds to and induces several genes containing an HIF-1 consensus-binding site, including vascular endothelial growth factor and several glycolytic enzymes. Through differential screening of a human synovial fibroblast cDNA library, we identified HIF-1α as a clone up-regulated by IL-1. The mRNA for HIF-1α subunit was increased 3–4-fold by Northern blot analysis after cells had been incubated for 3h in the presence of IL-1. In addition, IL-1 increased the binding of the heterodimer HIF-1 to the HIF consensus sequence. These results suggest that HIF-1 might have a role in inflammation, possibly in attempting to re-establish homoeostasis.
Interleukin 1 induces hypoxia-inducible factor 1 in human gingival and synovial fibroblasts
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Ruth D. THORNTON, Pamela LANE, Ruth C. BORGHAEI, Elizabeth A. PEASE, Jaime CARO, Eugene MOCHAN; Interleukin 1 induces hypoxia-inducible factor 1 in human gingival and synovial fibroblasts. Biochem J 15 August 2000; 350 (1): 307–312. doi: https://doi.org/10.1042/bj3500307
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