Epiglycanin is a mucin-type glycoprotein present at the surface of TA3Ha mouse mammary tumour cells. It is a long rod-like glycoprotein with a molecular mass of 500kDa. Its function has not yet been established but its overexpression can affect cell–cell and cell–matrix adhesion. To understand better the biological function of epiglycanin, we have studied the biochemical structure and biosynthesis of epiglycanin in TA3Ha cells. Pulse–chase labelling experiments with [3H]threonine revealed an early precursor with a molecular mass of approx. 300kDa containing approx. 5–10kDa of N-linked glycans. The precursor was gradually converted into a high-molecular-mass mature form, owing mainly, if not entirely, to O-glycosylation. The mature molecule consists of two major glycoforms that differ in sialylation. Unlike secreted mucins, epiglycanin did not form cysteine-bound multimers, providing further evidence that epiglycanin belongs to the class of membrane-associated mucins. The mature form, but not the precursor form, is shed from the cell surface. The half-life of epiglycanin on the cell surface was found to be approx. 60h. These results provide the first detailed analysis of the biochemical structure and biosynthesis of epiglycanin.
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January 2001
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Research Article|
December 18 2000
Biosynthesis and shedding of epiglycanin: a mucin-type glycoprotein of the mouse TA3Ha mammary carcinoma cell
Torunn THINGSTAD;
Torunn THINGSTAD
1Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
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Hans L. VOS;
Hans L. VOS
1Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
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John HILKENS
John HILKENS
1
1Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
1To whom correspondence should be addressed (e-mail jhi@nki.nl).
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Publisher: Portland Press Ltd
Received:
April 12 2000
Revision Received:
September 19 2000
Accepted:
October 16 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2001
2001
Biochem J (2001) 353 (1): 33–40.
Article history
Received:
April 12 2000
Revision Received:
September 19 2000
Accepted:
October 16 2000
Citation
Torunn THINGSTAD, Hans L. VOS, John HILKENS; Biosynthesis and shedding of epiglycanin: a mucin-type glycoprotein of the mouse TA3Ha mammary carcinoma cell. Biochem J 1 January 2001; 353 (1): 33–40. doi: https://doi.org/10.1042/bj3530033
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