Many glycoside hydrolases, which degrade long-chain carbohydrate polymers, possess distinct catalytic modules and non-catalytic carbohydrate-binding modules (CBMs). On the basis of conserved protein secondary structure, we describe here the identification and experimental characterization of novel type of mannanase-associated mannan-binding module and also characterization of two CBM family 4 laminarinase-associated β-glucan-binding modules. These modules are predicted to belong to a superfamily of CBMs which include families 4, 16, 17, 22 and a proposed new family, family 27.

Abbreviations used: CBD, cellulose-binding domain; CBM, carbohydrate-binding module; GH, glycoside hydrolase; GST, glutathione S-transferase; HE, hydroxyethyl; CM, carboxymethyl; LBG, locust bean gum; PBD, polysaccharide-binding domain; XBM, xylan-binding module.

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