The transcription factor nuclear factor κB (NF-κB) plays a pivotal role in inflammatory processes through induction of adhesion molecules and chemokines. The zinc finger molecule A20 is an important negative regulator of NF-κB. The mechanism utilized by A20 is not fully understood, but A20 has been shown to bind to tumour-necrosis-factor-receptor-associated factor (TRAF) molecules, which are necessary for pro-inflammatory cytokine signalling. We report two novel genes, Cezanne (cellular zinc finger anti-NF-kB) and TRABID (TRAF-bnding domain), with sequence similarity to A20. Co-immunoprecipitation studies indicated that TRAF6 was able to interact with both Cezanne and TRABID. In contrast, reporter gene experiments revealed a specific ability of Cezanne to down-regulate NF-κB. It is likely, therefore, that Cezanne participates in the regulation of inflammatory processes.
Abbreviations used: NF-κB, nuclear factor κB; IκB, inhibitory κB; TNF, tumour necrosis factor; TRAF, TNF-receptor-associated factor; TNFR1, TNF receptor 1; IL, interleukin; RACE, rapid amplification of cDNA ends; HRP, horseradish peroxidase; HA, haemagglutinin; GFP, green fluorescent protein; RT, reverse transciption; FLAG epitope, DYKDDDDK; Cezanne, cllular zinc finger anti-NF-kB; TRABID, TRAF-bnding domain.
The nucleotide sequence data reported have been deposited in the DDBJ, EMBL, GenBank® and GSDB Nucleotide Sequence Databases under the accession numbers AJ293573 and AJ252060.