We recently proposed a co-operative model for the influence of calcium and ADP on scallop (Argopecten irradians) muscle heavy meromyosin (scHMM), in which scHMM exists in two conformations (designated ‘off’ and ‘on'), and calcium and ADP are allosteric effectors of the equilibrium between the off and on conformations [Nyitrai, Szent-Gyorgyi and Geeves (2002) Biochem. J. 365, 19—30]. Here we examine the influence of actin on scHMM. In the absence of nucleotide, both heads of scHMM bind very tightly to actin, independent of the presence of calcium. In the absence of calcium, ADP dissociates scHMM from actin completely, and little evidence of ternary complex formation can be found (actin affinity >20μM). The off state of scHMM therefore does not interact with actin. In the presence of calcium, ADP and actin lower each other's affinity for scHMM by 30—50-fold, although both heads remain strongly attached to actin (actin affinity 0.17μM). Detailed analysis suggests that the second head contributes far more to the overall binding energy than is the case for mammalian skeletal muscle HMM. This is consistent with a different stereochemical relationship between the two heads in scallop and mammalian HMM molecules.

Abbreviations used: mant, 2′(3)-O-(N-methylanthraniloy); pyr-actin, pyrene-labelled actin; scHMM, heavy meromyosin from scallop (Argopecten irradians) striated muscle; S1, myosin subfragment 1; [X]F, concentration of X established after mixing the reactants in the stopped-flow apparatus; [X]I, concentration of X established before mixing the reactants in the stopped-flow apparatus (initial concentration).

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