Volume 392 (2005), pp. 665–674

1. There is an error in this sentence (page 671, left-hand column, first paragraph). The correct version is as follows:

These results suggest that hST8Sia VI prefers Neu5Acα2-3Galβ1-3GalNAc-R or Neu5Acα2-6GalNAc-R found in the O-glycans and also is highly sensitive to the underlying sequences.

2. There is an error in Table 1; the correct version appears below:

Table 1
Comparison of the acceptor substrate specificity of hST8Sia VI and m (mouse) ST8Sia VI

Various acceptor substrates were incubated in the standard assay mixture using transfected Sf-9 cells as an enzyme source. Each substrate was used at a concentration of 1 mM for arylglycosides, 2 mg/ml for glycoproteins and 1 mM for glycolipids. The specific [14C]Neu5Ac incorporation resulting from recombinant hST8Sia VI activity was estimated by subtracting the background sialyltransferase activities of mock transfected cells from the radioactivity in the presence of exogenous acceptor. Relative rates were calculated as a percentage of the incorporation of sialic acid onto fetuin. A value of 0 indicates <1%; nd, not determined; Cer, ceramide.

  Relative rate (%) 
Acceptor Structures hST8Sia VI mST8Sia VI* 
Glycoproteins    
 Fetuin Neu5Acα2-3Galβ1-3GalNAcα1-O-Ser/Thr   
 Neu5Acα2-3Galβ1-3[Neu5Acα2-6]GalNAcα1-O-Ser/Thr   
 Neu5Acα2-6(3)Galβ1-4GlcNAc-R 100 (0.15)† 100 (0.206)† 
 Asialofetuin Galβ1-3GalNAcα1-O-Ser/Thr and Galβ1-4GlcNAc-R 10 
 α1-Acid glycoprotein Neu5Acα2-6Galβ1-4GlcNAc-R 15 
 Asialo-α1-acid glycoprotein Galβ1-4GlcNAc-R 
 BSM GlcNAcβ1-3[Neu5Acα2-6]GalNAcα1-O-Ser/Thr   
 Neu5Acα2-6GalNAcα1-O-Ser/Thr 350 375 
 Asialo-BSM GlcNAcβ1-3GalNAcα1-O-Ser/Thr   
 GalNAcα1-O-Ser/Thr 15 
 OSM Neu5Acα2-6GalNAcα1-O-Ser/Thr 334 nd 
 Asialo-OSM GalNAcα1-O-Ser/Thr 12 nd 
Glycolipids    
 GM3 Neu5Acα2-3Galβ1-4Glcβ1-1′Cer 13 
 GD3 Neu5Acα2-8Neu5Acα2-3Galβ1-4Glcβ1-1′Cer 
 GD1a Neu5Acα2-3Galβ1-3GalNAcβ1-4[Neu5Acα2-3]Galβ1-4Glcβ1-1′Cer 12 
 GD1b Galβ1-3GalNAcβ1-4[Neu5Acα2-8Neu5Acα2-3]Galβ1-4Glcβ1-1′Cer 11 
 GT1b Neu5Acα2-3Galβ1-3GalNAcβ1-4[Neu5Acα2-8Neu5Acα2-3]Galβ1-4Glcβ1-1′Cer 25 1.1 
Arylglycosides    
 Galβ1-3GlcNAcβ-octyl 11 nd 
 Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα-octyl nd 
 Neu5Gcα2-3Galβ1-3GalNAcβ-octyl nd 
  Relative rate (%) 
Acceptor Structures hST8Sia VI mST8Sia VI* 
Glycoproteins    
 Fetuin Neu5Acα2-3Galβ1-3GalNAcα1-O-Ser/Thr   
 Neu5Acα2-3Galβ1-3[Neu5Acα2-6]GalNAcα1-O-Ser/Thr   
 Neu5Acα2-6(3)Galβ1-4GlcNAc-R 100 (0.15)† 100 (0.206)† 
 Asialofetuin Galβ1-3GalNAcα1-O-Ser/Thr and Galβ1-4GlcNAc-R 10 
 α1-Acid glycoprotein Neu5Acα2-6Galβ1-4GlcNAc-R 15 
 Asialo-α1-acid glycoprotein Galβ1-4GlcNAc-R 
 BSM GlcNAcβ1-3[Neu5Acα2-6]GalNAcα1-O-Ser/Thr   
 Neu5Acα2-6GalNAcα1-O-Ser/Thr 350 375 
 Asialo-BSM GlcNAcβ1-3GalNAcα1-O-Ser/Thr   
 GalNAcα1-O-Ser/Thr 15 
 OSM Neu5Acα2-6GalNAcα1-O-Ser/Thr 334 nd 
 Asialo-OSM GalNAcα1-O-Ser/Thr 12 nd 
Glycolipids    
 GM3 Neu5Acα2-3Galβ1-4Glcβ1-1′Cer 13 
 GD3 Neu5Acα2-8Neu5Acα2-3Galβ1-4Glcβ1-1′Cer 
 GD1a Neu5Acα2-3Galβ1-3GalNAcβ1-4[Neu5Acα2-3]Galβ1-4Glcβ1-1′Cer 12 
 GD1b Galβ1-3GalNAcβ1-4[Neu5Acα2-8Neu5Acα2-3]Galβ1-4Glcβ1-1′Cer 11 
 GT1b Neu5Acα2-3Galβ1-3GalNAcβ1-4[Neu5Acα2-8Neu5Acα2-3]Galβ1-4Glcβ1-1′Cer 25 1.1 
Arylglycosides    
 Galβ1-3GlcNAcβ-octyl 11 nd 
 Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα-octyl nd 
 Neu5Gcα2-3Galβ1-3GalNAcβ-octyl nd 
*

Data are from [32].

Actual activities are shown in brackets in pmol/h per μl.

3. There is an error in this sentence (page 672, right-hand column, first paragraph). The correct version is as follows:

However, in contrast with its recently characterized mouse counterpart [32], which also acts on the Neu5Acα2-3Galβ1-4Glc sequence of GM3 and on the Neu5Acα2-6Galβ sequences such as those found in N-glycosylproteins, the hST8Sia VI works almost exclusively on O-glycans.