Helicobacter pylori has a dynamic R-M (restriction–modification) system. It is capable of acquiring new R-M systems from the environment in the form of DNA released from other bacteria or other H. pylori strains. Random mutations in R-M genes can result in non-functional R-M systems or R-M systems with new properties. hpyAVIAM and hpyAVIBM are two solitary DNA MTase (methyltransferase) genes adjacent to each other and lacking a cognate restriction enzyme gene in H. pylori strain 26695. Interestingly, in an Indian strain D27, hpyAVIAM–hpyAVIBM encodes a single bifunctional polypeptide due to insertion of a nucleotide just before the stop codon of hpyAVIBM and, when a similar mutation was made in hpyAVIAM–hpyAVIBM from strain 26695, a functional MTase with an N-terminal C5-cytosine MTase domain and a C-terminal N6-adenine MTase domain was constructed. Mutations in the AdoMet (S-adenosylmethionine)-binding motif or in the catalytic motif of M.HpyAVIA or M.HpyAVIB selectively abrogated the C5-cytosine or N6-adenine methylation activity of M.HpyAVIA–M.HpyAVIB fusion protein. The present study highlights the ability of H. pylori to evolve genes with unique functions and thus generate variability. For organisms such as H. pylori, which have a small genome, these adaptations could be important for their survival in the hostile host environment.
Skip Nav Destination
Article navigation
February 2011
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
January 14 2011
A nucleotide insertion between two adjacent methyltransferases in Helicobacter pylori results in a bifunctional DNA methyltransferase
Ritesh Kumar;
Ritesh Kumar
1
1Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India
1To whom correspondence should be addressed (email rithesh@biochem.iisc.ernet.in).
Search for other works by this author on:
Desirazu N. Rao
Desirazu N. Rao
1Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 11 2010
Revision Received:
November 23 2010
Accepted:
November 29 2010
Accepted Manuscript online:
November 29 2010
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 433 (3): 487–495.
Article history
Received:
October 11 2010
Revision Received:
November 23 2010
Accepted:
November 29 2010
Accepted Manuscript online:
November 29 2010
Citation
Ritesh Kumar, Desirazu N. Rao; A nucleotide insertion between two adjacent methyltransferases in Helicobacter pylori results in a bifunctional DNA methyltransferase. Biochem J 1 February 2011; 433 (3): 487–495. doi: https://doi.org/10.1042/BJ20101668
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.