Using the ROS (reactive oxygen species)-sensitive fluorescent dyes dichlorodihydrofluorescein and dihydroethidine, previous studies yielded opposite results about the glucose regulation of oxidative stress in insulin-secreting pancreatic β-cells. In the present paper, we used the ratiometric fluorescent proteins HyPer and roGFP1 (redox-sensitive green fluorescent protein 1) targeted to mitochondria [mt-HyPer (mitochondrial HyPer)/mt-roGFP1 (mitochondrial roGFP1)] to monitor glucose-induced changes in mitochondrial hydrogen peroxide concentration and glutathione redox state in adenovirus-infected rat islet cell clusters. Because of the reported pH sensitivity of HyPer, the results were compared with those obtained with the mitochondrial pH sensors mt-AlpHi and mt-SypHer. The fluorescence ratio of the mitochondrial probes slowly decreased (mt-HyPer) or increased (mt-roGFP1) in the presence of 10 mmol/l glucose. Besides its expected sensitivity to H2O2, mt-HyPer was also highly pH sensitive. In agreement, changes in mitochondrial metabolism similarly affected mt-HyPer, mt-AlpHi and mt-SypHer fluorescence signals. In contrast, the mt-roGFP1 fluorescence ratio was only slightly affected by pH and reversibly increased when glucose was lowered from 10 to 2 mmol/l. This increase was abrogated by the catalytic antioxidant Mn(III) tetrakis (4-benzoic acid) porphyrin but not by N-acetyl-L-cysteine. In conclusion, due to its pH sensitivity, mt-HyPer is not a reliable indicator of mitochondrial H2O2 in β-cells. In contrast, the mt-roGFP1 fluorescence ratio monitors changes in β-cell mitochondrial glutathione redox state with little interference from pH changes. Our results also show that glucose acutely decreases rather than increases mitochondrial thiol oxidation in rat β-cells.
Glucose stimulation of insulin secretion by pancreatic β-cells plays a critical role in blood glucose homoeostasis. This acute glucose effect depends on the acceleration of its oxidation by glycolysis and the mitochondrial Krebs cycle, with a consequent increase in the production of various metabolic coupling factors, including NAD(P)H and ATP (reviewed in ). This is followed by plasma membrane depolarization, opening of voltage-dependent Ca2+ channels, Ca2+ influx and a rise in the free cytosolic Ca2+ concentration ([Ca2+]c) that triggers insulin granule exocytosis (reviewed in ). In addition, metabolic coupling factors derived from glucose oxidation amplify the efficacy of cytosolic Ca2+ on exocytosis and contribute to the acute stimulation of proinsulin biosynthesis and, in the long term, to the maintenance of the β-cell phenotype (reviewed in [2,3]).
Although the link between the acceleration of glucose metabolism and plasma membrane depolarization involves a rise in the cytosolic ATP/ADP ratio that closes ATP-sensitive K+ channels, other mechanisms may also be operating . Among these, it has been suggested that an increase in the mitochondrial production of H2O2 plays a critical role in glucose stimulus-secretion coupling in β-cells . On the other hand, oxidative stress together with endoplasmic reticulum stress may contribute to the alteration of β-cell function, survival and gene expression induced by chronic exposure to high glucose and lipid concentrations in the context of Type 2 diabetes (reviewed in [6,7]). Such oxidative stress could result from various mechanisms, including increased production of superoxide (coupled to H2O2 formation) by the mitochondrial electron transport chain, activation of the NADPH oxidase complex and protein glycation [8–10]. That β-cell oxidative stress is increased by high glucose concentrations was demonstrated using classical detection of oxidative markers such as 4-OHnonenal and 8-OH-deoxyguanosine on pancreatic sections from Type 2 diabetic rodents, and by measuring the oxidation of ROS (reactive oxygen species)-sensitive fluorescent dyes, like dichlorodihydrofluorescein and dihydroethidine, in isolated islets [11,12]. Using the same tools, however, Martens et al. demonstrated that the β-cell ROS concentration is high at low glucose concentrations and decreases following nutrient stimulation of mitochondrial metabolism. This is likely because of an increase in NAD(P)H production, hence in ROS scavenging capacity , the mechanism of which may include activation of the pentose phosphate pathway . Interestingly, the increase in ROS at low glucose concentrations was accompanied by an increase in β-cell apoptosis, and both events were reduced by MnTBAP [Mn(III) tetrakis (4-benzoic acid) porphyrin] [13,15]. This antioxidant is usually considered a SOD (superoxide dismutase), a catalase-mimetic and a potent inducer of the antioxidant enzyme haem oxygenase 1 . However, it has been shown that pure MnTBAP mainly reduces peroxinitrite and carbonate radicals while commercial MnTBAP (as used in the present study) also contains traces of SOD-mimetic [17,18].
Several methods are frequently used to measure ROS production in living cells, the most popular being the oxidation of Amplex Red and dichlorodihydrofluorescein. Despite their high sensitivity, these probes do not detect specific ROS, undergo rapid photo-oxidation that prevents their use in dynamic measurements and cannot be targeted to identify the subcellular sites of ROS production (reviewed in ). In contrast, new genetically encoded probes have been developed that allow sensitive and compartment-specific dynamic measurements of ROS production in living cells . Among them, HyPer is a chimaeric protein composed of the H2O2-sensing regulatory domain of the bacterial transcription factor OxyR and a cpYFP (circularly permuted yellow fluorescent protein) insertion . Although HyPer is as pH-sensitive as other cpYFP-based probes [21–23], it was recently used to test the subcellular source of oxidative stress in β-cells exposed to saturated fatty acids . In comparison, roGFP (redox-sensitive green fluorescent protein) is not a specific sensor of H2O2, but is a good indicator of changes in the thiol/disulfide intracellular redox equilibrium .
In view of the conflicting results about the glucose regulation of oxidative stress in β-cells, and because of its possible role in β-cell failure in Type 2 diabetes, we chose to use genetically encoded probes to readdress these questions. In the present study, we tested the adequacy of mitochondria-targeted HyPer [mt-HyPer (mitochondrial HyPer)] and roGFP1 [mt-roGFP1 (mitochondrial roGFP1)] to monitor glucose-induced changes in rat β-cell mitochondrial H2O2 production and glutathione redox status. To evaluate the influence of mitochondrial pH on these probes, the results were compared with those obtained with the pH sensors mt-AlpHi and mt-SypHer [26,27].
MATERIAL AND METHODS
Na+-azide, NH4Cl and Na+-acetate were from Sigma. MnTBAP was purchased from Alexis Biochemicals and NAC (N-acetyl-L-cysteine) from Merck. Restriction enzymes were from Fermentas.
The plasmid encoding mt-Hyper  was obtained from Evrogen, and that encoding mt-SypHer, an H2O2-insensitive form of HyPer in which Cys199 was replaced by a serine residue , was a gift from N. Demaurex (Department of Cell Physiology and Metabolism, University of Geneva Medical School, Geneva, Switzerland). The plasmid encoding doxycycline-inducible mt-AlpHi was described previously . The plasmid encoding mt-roGFP1 (ro1 with mitochondrial targeting sequence in pEGFP-N1, pRA306)  was obtained from S.J. Remington (University of Oregon, Eugene, OR, U.S.A.).
The generation of an adenovirus encoding the fluorescent protein DsRed under the control of the RIP (rat insulin promoter) was described previously . Adenoviruses encoding mt-Hyper and mt-roGFP1 were generated and amplified using the AdEasy XL Adenoviral Vector System. Briefly, the cDNA encoding mt-Hyper or mt-roGFP1 were inserted into the pShuttle-CMV vector, and then re-cloned in the adenoviral backbone plasmid pAdEasy. The resultant pAd-mt-HyPer and pAd-mt-roGFP1 plasmids were digested with PacI and transfected into HEK-293 cells (human embryonic kidney cells) to generate adenovirus particles. Adenoviruses encoding RIP-mt-AlpHi and mt-SypHer were generated and amplified using the Adeno-X Adenoviral Expression System (Clontech). Briefly, the promoter sequence for doxycycline-inducible expression (tetON CMVmin) was removed from the pTRE Shuttle vector (Clontech) and replaced by the RIP followed by mt-AlpHi, or by a CMV promoter together with mt-SypHer. The resulting constructs were re-cloned into the pAdenoX vector. The vector was linearized using PacI to be used for transfection of HEK-293 cells to generate adenovirus particles. All adenoviruses were amplified in HEK-293 cells and purified on a CsCl gradient.
Islet isolation and adenoviral infection
All animal experimentations were approved by the local Institutional Committee on Animal Experimentation of the Faculty of Medicine of the Université Catholique de Louvain (Project UCL/MD/2009/009). Islets were isolated by collagenase digestion of the pancreas of male Wistar rats (180–200 g) as previously described . They were washed and hand-picked under a stereomicroscope to ensure high purity of the preparation, then dispersed into small cell clusters using trypsin and gentle pipetting in a Ca2+-free medium. These clusters were plated on glass coverslips and cultured at 37°C in the presence of 5% CO2 with RPMI 1640 medium (Invitrogen) containing 10 mmol/l glucose (G10), 10% fetal bovine serum, penicillin and streptomycin. After overnight culture, cells were infected with 1–1.5 μl of RIP-DsRed, mt-HyPer, mt-roGFP1, mt-SyPher, or RIP-mt-Alphi encoding adenovirus. They were then cultured for 2 days before fluorescence measurements.
Static fluorescence measurements
At 2 days after co-infection of islet cell clusters with RIP-DsRed plus mt-HyPer, mt-roGFP1, mt-SypHer or RIP-mt-AlpHi, cell nuclei were stained for 20 min with the DNA-binding fluorescent dye Hoechst 33342. The fluorescence was analysed using an EVOS microscope (Bothell) and the following λex/λem (nm): Hoechst 33342 (357/447); DsRed (531/593); mt-HyPer, mt-roGFP1 and mt-SypHer (470/525). The images were captured using a ×40 objective. In other experiments, the mitochondrial expression of the various probes was documented with a Nikon Eclipse TE2000-E inverted microscope equipped with a confocal QLC100 spinning disk (Visitech International), using a ×60 oil immersion objective.
Dynamic fluorescence measurements
After culture, the coverslip was mounted at the bottom of a 37°C temperature-controlled chamber place on the stage of an inverted microscope. Cell clusters were perifused at a flow rate of ~1 ml/min with a bicarbonate-buffered Krebs solution containing: 120 mmol/l NaCl, 4.8 mmol/l KCl, 2.5 mmol/l CaCl2, 1.2 mmol/l MgCl2, 24 mmol/l NaHCO3, 1 g/l BSA (fraction V; Roche) and various glucose concentrations and test substances. This solution was continuously gassed with O2/CO2 (94/6) to maintain pH ~7.4. Excitation light at appropriate wavelengths was produced by a monochromator: 495/420 nm for mt-HyPer, 400/480 nm for mt-roGFP1, 480/430 nm for mt-SypHer and 490 nm for RIP-mt-AlpHi. For all probes, a 535 nm emission filter was used. The cells were analysed with a ×40 objective and images were acquired every 30 s. Results are shown as means±S.E.M. for the indicated number of cells and experiments. In each Figure, the statistical significance of changes in fluorescence ratio (or absolute levels for mt-AlpHi) between selected time points (1 min average) was assessed by one-way ANOVA for repeated measurements followed by a Newman–Keuls test.
The CMV promoter drives the expression of the mitochondrial sensors preferentially in β-cells
Dissociated rat islet cells were infected with adenoviruses carrying transgenes expressed under the control of the CMV promoter. The proportion of β-cells and non-β-cells expressing CMV-driven mt-roGFP1, mt-HyPer and mt-SypHer was determined by co-infection with an adenovirus encoding DsRed under the control of the RIP (RIP-DsRed) (Figure 1) . On average, 84% of mt-HyPer-positive cells were also positive for RIP-DsRed, while double positivity was observed in 90% of mt-roGFP1-positive cells and 80% of mt-SypHer-positive cells. These results demonstrate that mt-HyPer, mt-roGFP1 and mt-SypHer are preferentially expressed in β-cells versus non-β-cells. In addition, the fluorescence intensity of the probes was lower in RIP-DsRed-negative versus RIP-DsRed-positive cells (compare the cluster and the single non-β-cell indicated by an arrow in Figure 1). Therefore, to further reduce the likelihood of studying non-β-cells, changes in probe fluorescence ratios were only measured in brightly fluorescent cells within clusters. As expected, all RIP-mt-AlpHi-positive cells were also positive for RIP-DsRed (results not shown). Confocal imaging of mt-HyPer, mt-roGFP1 and mt-SypHer expressing cells confirmed that these probes correctly targeted to mitochondria (Figure 1, right-hand panels).
Expression of mt-HyPer, mt-roGFP1 and mt-SypHer with RIP-DsRed in rat islet cell clusters and their intracellular localization
Glucose-induced changes in mt-Hyper signal
To evaluate whether glucose acutely increases mitochondrial H2O2 production in rat β-cells, we initially tested the effects of glucose on mt-HyPer fluorescence ratio in perifused islet cell clusters. When the medium contained 10 mmol/l glucose (G10) throughout the experiment, mt-HyPer fluorescence ratio tended to decrease slowly for at least 1 h (Figure 2A). As expected, addition of 100 μmol/l H2O2 to G10 rapidly increased this ratio 2–3-fold. When the glucose concentration was increased stepwise from G2 to G30, mt-HyPer fluorescence ratio significantly increased by approximately 20% between G2 and G10, but did not further increase between G10 and G30 (Figure 2B). On the contrary, when glucose was reduced from G10 to G2, mt-HyPer fluorescence ratio transiently increased and then decreased to a lower level (Figure 2C). On return to G10, mt-Hyper fluorescence ratio increased back to its initial level. At first sight, these glucose-induced changes in mt-HyPer fluorescence ratio could be taken as evidence that the glucose stimulation of pancreatic β-cells increases mitochondrial H2O2 concentration from G2 to G10, with no further increase above G10. However, the catalytic antioxidant MnTBAP and the free radical scavenger NAC failed to suppress glucose-induced changes of mt-HyPer fluorescence (Figures 2D–2E). These findings suggested that, during glucose stimulation of rat islet β-cells, mt-HyPer fluorescence is altered by a mitochondrial parameter other than the H2O2 concentration.
Glucose-induced changes in mt-HyPer fluorescence ratio in rat islet cell clusters
Glucose-induced changes in mt-HyPer fluorescence ratio mainly reflect changes in mitochondrial pH
It has been reported that, like other fluorescent probes derived from circularly permuted fluorescent proteins, HyPer is sensitive to changes in intracellular pH . The similarity between the glucose-induced changes in the mt-HyPer signal (see Figure 2) and mitochondrial pH increase measured with mt-AlpHi in rat islets  suggested that these changes in mt-HyPer fluorescence ratio in β-cells could reflect changes in mitochondrial pH rather than H2O2.
To test this hypothesis, we first measured the effects of cell acidification induced by Na+-acetate and of cell alkalinization with NH4Cl in islet cells expressing mt-HyPer and compared the results with those obtained in cells expressing the mitochondrial pH-sensor mt-AlpHi or mt-SypHer. The latter pH probe is derived from HyPer by replacing the first H2O2-sensing Cys199 by a serine residue, as described previously . As shown in Figures 3(A), 3(C) and 3(E), Na+-acetate similarly decreased while NH4Cl similarly increased mt-HyPer fluorescence ratio, mt-AlpHi fluorescence intensity and mt-SypHer fluorescence ratio. As expected, subsequent addition of 100 μmol/l H2O2 to G10 only increased mt-HyPer fluorescence ratio without affecting mt-SypHer or mt-AlpHi signals (Figures 3A, 3C and 3E).
pH sensitivity of mt-Hyper, mt-SypHer and mt-AlpHi fluorescence signal in rat islet cell clusters
Inhibitors of the electron transport chain are considered to increase mitochondrial ROS production. It has, however, also been reported that they induce mitochondrial matrix acidification in pancreatic β-cells and in other cell types [26,27]. In agreement with the latter effect, addition of 3 mmol/l azide to G10 similarly decreased mt-HyPer fluorescence ratio, mt-AlpHi fluorescence intensity and mt-SypHer fluorescence ratio (Figures 3B, 3D and 3F). This effect of azide was completely reversible.
We finally tested the effects of glucose on β-cell mitochondrial pH in our experimental system. In agreement with earlier data showing that glucose stimulation triggers mitochondrial matrix alkalinization in rat islets , both mt-SypHer and mt-AlpHi signals increased when the glucose concentration was raised from G2 to G10. A further increase was observed between G10 and G30 with mt-AlpHi but not mt-SypHer (Figure 4). The similarity of the latter changes with those observed in mt-HyPer-expressing cells strongly supported our hypothesis that glucose-induced changes in mt-HyPer fluorescence ratio in β-cells better reflect changes in mitochondrial pH than in H2O2 concentration.
Effects of glucose on mt-SypHer and mt-AlpHi fluorescence signal in rat islet cell clusters
Acute glucose-induced changes in mt-roGFP1 fluorescence
As mt-HyPer proved highly sensitive to changes in intracellular pH, we decided to test whether roGFP1 targeted to mitochondria (mt-roGFP1) could be used to monitor glucose-induced changes in β-cell mitochondrial redox state independently from changes in pH. In contrast with HyPer, the redox-sensitive probe mt-roGFP1 does not directly sense H2O2 but rather reflects changes in the thiol/disulfide (GSH/GSSG) ratio [20,28]. The pH sensitivity of mt-roGFP1 was first assessed using Na+-acetate and NH4Cl. As shown in Figure 5(A), mt-roGFP1 fluorescence ratio was not affected by Na+-acetate and only slightly increased upon cell alkalinization with NH4Cl, demonstrating a very low pH sensitivity of this probe. Consistent with these findings, mt-roGFP1 fluorescence ratio was not significantly affected by azide (Figure 5B). In contrast, mt-roGFP1 signals rapidly increased upon addition of 100 μmol/l H2O2, confirming that the probe can detect an increase in H2O2 concentration in the mitochondrial matrix. We next tested the effects of glucose in islet cell clusters expressing mt-roGFP1. As shown in Figures 6(A) and 6(B), the mt-roGFP1 fluorescence ratio tended to increase slowly and regularly during perifusion with G10 and was unaffected by a rise in glucose concentration from G10 to G30. However, in contrast with results obtained with the pH sensors mt-AlpHi and mt-SyPher, mt-roGFP1 fluorescence ratio reversibly increased when glucose was acutely lowered from G10 to G2 and raised back to G10 (Figure 6C). Remarkably, the effect of G2 was almost fully suppressed by addition of MnTBAP, but not NAC, to the perifusion medium (Figures 6D and 6E), indicating that glucose stimulation acutely suppresses rather than increases thiol oxidation in β-cell mitochondria.
Effects of cell acidification and alkalinization in rat islet cell clusters expressing mt-roGFP1
Effects of glucose in rat islet cell clusters expressing mt-roGFP1
In the present study, we demonstrate that, due to its high pH sensitivity, mt-HyPer is not a reliable tool to measure nutrient-induced changes in mitochondrial H2O2 production in insulin-secreting pancreatic β-cells. In contrast, mt-roGFP1, which displays a low pH sensitivity, can be used to monitor nutrient-induced changes in β-cell mitochondrial redox status. The results obtained with mt-roGFP1 suggest that lowering the glucose concentration from 10 to 2 mmol/l acutely increases thiol (most likely GSH) oxidation in the mitochondrial matrix of β-cells, whereas increasing glucose from 10 to 30 mmol/l does not detectably affect it within 40 min.
In comparison with small chemical fluorescent probes, such as dichlorodihydrofluorescein and dihydroethidine that distribute throughout the cell and do not measure a specific type of ROS species , genetically encoded redox-sensitive probes derived from fluorescent proteins are attractive probes as they allow monitoring of compartment-specific redox changes (reviewed in ). In their initial report on HyPer, Belousov et al.  demonstrated that the probe was sensitive to small changes in intracellular H2O2 while being insensitive to other oxidants such as superoxide, peroxynitrite and oxidized glutathione. However, as expected for probes derived from circularly permuted fluorescent proteins , HyPer fluorescence ratio was also reported to be sensitive to pH variations as low as 0.2 pH units, the fluorescence ratio of its reduced form displaying an ~1.8-fold increase upon alkalinization from pH 7.1 to 7.4 and a ~30% decrease upon acidification from pH 7.1 to 6.9 . In agreement, concentrations of Na+-acetate and NH4Cl previously reported to affect mitochondrial pH by ~0.2 units in HeLa cells  respectively decreased by ~20% and increased ~1.8-fold the mt-HyPer fluorescence ratio in rat islet cells. Nevertheless, HyPer has been used to monitor H2O2 production in many cell types under physiological and pathophysiological conditions, including in clonal insulin-producing cells and primary β-cells cultured under lipotoxic conditions, without adequately assessing the possible confounding effects of changes in pH [20,21,24].
It has previously been shown that the stimulation of pancreatic islets with glucose and other nutrients induce rapid changes in their cytosolic and mitochondrial pH, the latter being of larger amplitude than the former [27,33,34]. Using 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, Shepherd et al. [33,34] demonstrated that the intracellular pH of mouse β-cells is slightly acidic (pH 6.9) in the absence of glucose, and that their stimulation with glucose and other mitochondrial substrates induces a net alkalinization of ~0.2 pH units that results from a balance between the alkalinizing action of an increase in mitochondrial metabolism and the acidifying action of a rise in cytosolic calcium concentration. Using the mt-AlpHi pH probe, it was shown that the mitochondrial matrix of rat pancreatic β-cells is unusually acidic at a low glucose concentration (pH ~7.25) and that glucose stimulation alkalinizes the mitochondrial matrix to a value close to that measured in other cell types (pH ~7.7) . This alkalinization, which was correlated with the increase in cytosolic ATP concentration, was proposed to control mitochondrial ATP generation during sustained stimulation of insulin secretion .
We observed that, although mt-HyPer is capable of detecting an increase in β-cell mitochondrial H2O2, the changes in mt-HyPer fluorescence ratio induced by glucose, Na+-acetate, NH4Cl and azide were almost identical with those observed using the pH sensors mt-AlpHi and mt-SypHer. In addition, the glucose-induced changes in mt-HyPer fluorescence ratio were not affected by the antioxidants MnTBAP and NAC, suggesting that they do not result from changes in H2O2 concentration. Altogether, our results indicate that the unusual change in mitochondrial matrix pH observed in β-cells stimulated with nutrients combined with the high pH sensitivity of HyPer precludes the use of that probe to measure changes in H2O2 concentration under these conditions. Whether this probe can be used in the cytosol of β-cells was not tested in the present study, but the amplitude of glucose-induced changes in cytosolic pH is large enough to affect the HyPer fluorescence ratio . Therefore we suggest that, when using HyPer in living cells, SypHer be systematically used to control for the possible confounding effect of changes in pH.
In comparison with HyPer, the fluorescent protein roGFP1 is not selective for H2O2, but specifically senses changes in the glutathione redox state, which results from the dynamic equilibrium between its oxidation and reduction. This probe therefore only indirectly senses an increase in H2O2 levels. However, roGFP1 has the advantage of being much less sensitive than HyPer to changes in pH between 6.0 and 8.0 , as confirmed by our data. To our knowledge, the use of roGFP1 in native β-cells or β-cell lines has not been reported yet. We observed that lowering the glucose concentration from 10 to 2 mmol/l rapidly and reversibly increased mt-roGFP1 fluorescence ratio and that this effect was prevented by the antioxidant MnTBAP but not NAC. In contrast, an increase in glucose concentration from 10 to 30 mmol/l did not detectably affect the mt-roGFP1 signal within 40 min of stimulation. These results question previous studies showing that glucose stimulation acutely increases β-cell ROS production [12,35]. On the contrary, they extend previous studies demonstrating that the oxidation of dichlorodihydrofluorescein and dihydroethidine in β-cells was high in the absence of glucose and rapidly decreased between 0 and 10 mmol/l glucose, but did not increase upon a rise in glucose from 10 to 20 mmol/l [13–15]. In these studies, the non-specific redox catalyst MnTBAP [17,18,36] significantly reduced β-cell ROS production and apoptosis induced by low glucose. In contrast, the free radical scavenger and GSH precursor NAC did not prevent the increase in mt-roGFP1 signal induced at low glucose, nor did it protect β-cells against apoptosis in these conditions (L.P. Roma, S.M. Pascal, J. Duprez and J.C. Jonas, unpublished work). The different efficacy of MnTBAP and NAC on both ROS production and apoptosis at low-glucose concentrations might be related to the type of ROS generated, their site of production or the ability of these compounds to reach the mitochondrial matrix. However, overexpression of cytosolic and mitochondrial antioxidant enzymes with defined ROS specificities will be required to correctly address these questions.
Previous studies have yielded conflicting results regarding the glucose regulation of β-cell ROS production (reviewed in ). The present study reinforces the view that acute lowering of the glucose concentration from 10 to 2 mmol/l reversibly increases β-cell ROS production and/or reduces their antioxidant defence [14,15,37]. The fact that high glucose (30 mmol/l) did not acutely increase mt-roGFP1 oxidation in islet cell clusters does not, however, exclude the possibility that high glucose acutely stimulates ROS production in a compartment from where it does not reach the mitochondrial matrix, or that it does so only transiently, thereby escaping detection by roGFP1. It also does not rule out the hypothesis that a long-term exposure to high glucose concentrations (30 compared with 10 mmol/l) could induce mitochondrial oxidative stress.
In conclusion, the high pH sensitivity of mt-HyPer prevents its use as a reliable indicator of glucose-induced changes in mitochondrial H2O2 production in rat β-cells. In contrast, mt-roGFP1 can be used to monitor changes in glutathione redox state in β-cell mitochondria independently from changes in pH. Our results also show that glucose acutely reduces rather than increases mitochondrial thiol oxidation in rat β-cells.
circularly permuted yellow fluorescent protein
human embryonic kidney
Mn(III) tetrakis (4-benzoic acid) porphyrin
rat insulin promoter
redox-sensitive green fluorescent protein
reactive oxygen species
Leticia Roma and Jean-Christophe Jonas conceived and designed the experiments; Leticia Roma and Jessica Duprez performed the experiments; Leticia Roma and Jean-Christophe Jonas analysed the results; Patrick Gilon and Andreas Wiederkehr contributed to reagents/materials/analysis tools; Leticia Roma, Hilton Takahashi and Jean-Christophe Jonas wrote the paper; Jessica Duprez, Patrick Gilon and Andreas Wiederkehr made corrections/suggestions to the paper.
We thank Denis Charlier for expert technical help and Nicolas Demaurex (Department of Cell Physiology and Metabolism, University of Geneva Medical School, Geneva, Switzerland) for providing the plasmid encoding SypHer.
This work was supported by the Fonds de la Recherche Scientifique Médicale (Belgium) [grant number 3.4516.09], the Interuniversity Poles of Attraction Program (P6/42)-Belgian Science Policy and the Société Francophone du Diabète. J.D. is a recipient of an F.R.I.A. (Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture) fellowship (Belgium). J.C.J. and P.G. are Research Directors of the Fonds de la Recherche Scientifique-FNRS (Belgium).
Present address: Nestlé Institute of Health Sciences, Lausanne, Switzerland.