NIH-12848 (NCGC00012848-02), a putative phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) inhibitor, was explored as a tool for investigating this enigmatic, low activity, lipid kinase. PI5P4K assays in vitro showed that NIH-12848 inhibited PI5P4Kγ with an IC50 of approximately 1 μM but did not inhibit the α and β PI5P4K isoforms at concentrations up to 100 μM. A lack of inhibition of PI5P4Kγ ATPase activity suggested that NIH-12848 does not interact with the enzyme's ATP-binding site and direct exploration of binding using hydrogen–deuterium exchange (HDX)-MS (HDX-MS) revealed the putative PI5P-binding site of PI5P4Kγ to be the likely region of interaction. This was confirmed by a series of mutation experiments which led to the identification of a single PI5P4Kγ amino acid residue that can be mutated to its PI5P4Ks α and β homologue to render PI5P4Kγ resistant NIH-12848 inhibition. NIH-12848 (10 μM) was applied to cultured mouse principal kidney cortical collecting duct (mpkCCD) cells which, we show, express PI5P4Kγ that increases when the cells grow to confluence and polarize. NIH-12848 inhibited the translocation of Na+/K+-ATPase to the plasma membrane that occurs when mpkCCD cells grow to confluence and also prevented reversibly their forming of ‘domes’ on the culture dish. Both these NIH-12848-induced effects were mimicked by specific RNAi knockdown of PI5P4Kγ, but not that of PI5P4Ks α or β. Overall, the data reveal a probable contribution of PI5P4Kγ to the development and maintenance of epithelial cell functional polarity and show that NIH-12848 is a potentially powerful tool for exploring the cell physiology of PI5P4Ks.
INTRODUCTION
The phosphatidylinositol 5-phosphate 4-kinases (PI5P4Ks) are a family consisting of three isoforms in mammals (α, β and γ), whose most probable function is currently believed to be the regulation of the levels of their substrate, PI5P [1,2]. Although the α and β isoforms have had some physiological and pathological functions assigned to them, including in gene regulation [3,4], stress responses [5], insulin signalling [6] and cancer [7,8], PI5P4Kγ is hardly understood at all. It is apparently ubiquitously expressed, but at very different levels between tissues, being particularly high in epithelial cells of the thick ascending limb of the kidney and collecting ducts cells [9] and in specific neurons in brain [10]. It apparently has a vesicular localization [9,10], may have a functional connection with Rho [11], shows a much lower enzymatic activity in vitro than the other PI5P4K isoforms [12] and can heterodimerize in vitro with PI5P4Kα [12] (note that extensive heterodimerization between PI5P4Ks α and β has been shown to occur in vivo [13,14]).
Specific inhibitors of enzymes can be useful tools in studying their function and kinase inhibitors are among those that have shown most promise as potential therapeutic agents. Recently, the characterization of inhibitors for PI5P4Kα [15] and PI5P4Kβ [16] have raised that hope for those isoforms, but the isoform specificity of neither inhibitor has yet been established and so far no such tools exist for PI5P4Kγ. Moreover, a challenge facing any kinase inhibitor, the great majority of which interact with the ATP-binding site of their target, is for it to have both sufficient specificity (because all kinase ATP-binding sites show some structural similarity) and potency (cellular concentrations of ATP are in the millimolar range, so nanomolar affinity of an inhibitor is often required for micromolar efficacy in a cell). The high affinity and specificity of the phosphoinositide 3-kinase (PI3K)-δ inhibitor PIK-39 that results from a remarkable induced fit into the ATP-binding site of its target protein [17] is one example of an ATP-binding site competitor that overcomes these issues. A potential approach for increasing the kinase specificity is to look for ATP-allosteric modulators, although in some cases (e.g. [18]) there are discrepancies between cell-based and isolated kinase inhibitory assays, making difficult the finding of this kind of inhibitor.
Herein, we report the characterization and use of a PI5P4Kγ-specific inhibitor NIH-12848 (full designation NCGC00012848-02), which we show interacts not with the ATP-binding site but with the region where PI5P probably binds, including the activation loop. We use the inhibitor to begin the first exploration, in a kidney epithelial cell line, of the function of PI5P4Kγ. Also, we show how we can mutate PI5P4Kγ so that it becomes insensitive to NIH-12848, opening the possibility of chemical biology to explore the functions of all three PI5P4Ks.
MATERIALS AND METHODS
Enzyme preparation and mutagenesis
Recombinant enzyme was prepared essentially as described previously [12]. Protein from PIP4K2C (UniGene 6280511) or associated mutants, cloned into the expression vector pGEX6P (GE Healthcare) was expressed and purified from Escherichia coli BL21(DE3). Cultures were induced with 0.4 mM IPTG and probe-sonicated in the presence of protease inhibitors. GST fusion proteins of PI5P4Kγ and PI5P4Kγ+, a mutant with specific activity close to that of the active PI5P4Kα isoform [12], were harvested by binding to glutathione sepharose beads (GE Healthcare) and cleaved in situ with 50 units of PreScission protease (GE Healthcare) for 4 h at 4°C. Purity was confirmed by SDS/PAGE and protein concentration determined by colorimetric assay (Bio-Rad). Site-directed mutagenesis using the QuikChange technique (Agilent Technologies) was used to generate clones from which mutant forms of PI5P4Kγ and PI5P4Kγ+ were produced (for mutagenesis primers see Supplementary Table S1).
Biochemical assays
Lipid kinase assays were performed as described previously [13]. Dipalmitoyl-PI5P (DiC16–PI5P) was purchased from Echelon Biosciences and after drying down in Eppendorf tubes was sonicated for 3 × 30 s in a Decon Ultrasonics sonicating bath. This lipid substrate (6 μM PI5P) and recombinant lipid kinase were added to the reaction mixture (200 μl of final volume) with 10 μCi [γ-32P]ATP and incubated at 30°C for 10–60 min. Lipids were extracted using an acidic phase-separation [19] and separated by 1D thin layer chromatography (2.8:4:1:0.6 chloroform:methanol:water:ammonia). Radiolabelled PtdIns(4,5)P2 spots were detected by autoradiography, extracted and counted with Ultima Gold XR scintillant (Packard) on a LS6500 scintillation counter (Beckman Coulter). Intrinsic ATPase activities of the enzymes were determined using the Transcreener ADP2 fluorescence polarization method (BellBrook Labs). A range of enzyme concentrations was assayed with ATP substrate (100 μM ATP, 60 min incubation at 22°C) and polarization units (mP) were read using a PHERAstar Plus microplate reader (BMG Labtech). Experimental values were interpolated from an ADP/ATP utilization standard curve and plotted using non-linear regression analysis with Prism 5.
Hydrogen–deuterium exchange MS
Hydrogen–deuterium exchange (HDX)-MS experiments were performed according to previously published protocols [20,21]. In brief, HDX reactions were initiated in HDX buffer [20 mM HEPES, pH 7.5, 50 mM NaCl, 2 mM Tris carboxyl ethyl phosphine (TCEP)] by the addition of 98% 2H2O solution (10 mM HEPES pH 7.5, 50 mM NaCl, 2 mM TCEP), to give a final concentration of 78% 2H2O. Final protein concentrations were 1 μM. For all reactions with inhibitor, there was a 10-fold excess of inhibitor over protein and this was allowed to incubate for 30 min on ice before initiation of HDX. Four time points of exchange were carried out (3, 30 and 300 s at 23°C and 3 s at 0°C) and were terminated by the addition of a quench buffer (1 M guanidine-HCl, 0.8% formic acid), followed by snap freezing in liquid nitrogen. Samples were stored at −80°C until mass analysis.
Measurement of deuterium incorporation
Protein samples were rapidly thawed and injected on to a UPLC (ultra performance liquid chromatography) column immersed in ice, as previously described [21]. The protein was run over two immobilized pepsin columns in series (Applied Biosystems; porosyme) at 200 μl/min for 3 min and collected over a van-guard pre-column trap (Waters). The trap was subsequently eluted in line with an Acquity 1.7 μm particle, 100 x 1 mm2 C18 UPLC column (Waters), using a gradient of 5%–36% buffer B (buffer A 0.1% formic acid, buffer B 100% acetonitrile) over 20 min. Eluent from the column was injected on to a Xevo QTOF (Waters) acquiring over a mass range from 350 to 1500 m/z, with an ESI source operated at a temperature of 225°C and a spray voltage of 2.5 kV.
Protein digestion, peptide identification and mass analysis
Peptide identification was done by running tandem MS/MS experiments using a 3%–35% B gradient over 120 min with a Xevo QTOF (Waters). This was supplemented with a 20 min gradient separation to identify and correct the retention time for all samples. The MS tolerance was set to 3 ppm with a MS/MS tolerance at 0.1 Da. The resulting MS/MS datasets were analysed with the Mascot search within Mascot distiller (Matrix Science). All peptides with a Mascot score >15 were analysed using HD-Examiner Software (Sierra Analytics). The full list of peptides was then manually validated by searching a non-deuterated protein sample's MS scan to test for the correct m/z state and check for the presence of overlapping peptides. Ambiguously identified peptides were excluded from all subsequent analysis. The first round of analysis and identification were performed automatically by the HD-Examiner software, but all peptides (deuterated and non-deuterated) were manually verified at every state and time point for the correct charge state, m/z range, presence of overlapping peptides and the expected retention time. All HDX–MS results are presented as relative levels of deuterium incorporation and no correction for back exchange is applied, because no fully deuterated protein sample could be obtained.
Cell culture
Immortalized mouse cortical collecting duct (mpkCCD) cells [22,23] were grown in defined medium [Dulbecco's Modified Eagle Medium (DMEM):Ham's F12 1:1 (v/v), 60 nM sodium selenate, 5 μg/ml transferrin, 2 mM glutamine, 50 nM dexamethasone, 1 nM tri-iodothyronine, 10 ng/ml epidermal growth factor, 5 μg/ml insulin, 20 mM D-glucose, 2% (v/v) fetal calf serum and 20 mM HEPES, pH 7.4] at 37°C in a 5% CO2, 95% air atmosphere. Medium was changed every 2 days and all studies were performed on cells that had been grown on plastic Petridishes or glass slides.
RNAi
Depletion of specific PI5P4K isoforms was achieved by transfecting cells with siRNAs specific to each PI5P4K isoform. The siRNAs were obtained from Thermo Scientific Dharmacon (ON-TARGETplus SMART pool). Control siRNAs were sequences that would not be recognized by any part of the mouse genome. mpkCCD cells were plated at a density of 6 × 104 per well (24-well/plate) 1 day before transfection, then were transfected with each siRNA at a final concentration of 25 pmol/ml using DharmaFECT 1 Transfection Reagent (Dharmacon). At 72-h post-transfection, a new round of transfection was performed and the cells were cultured for another 48 h.
Morphological and immunocytochemical studies
Confluent cells grown on plastic Petridishes were examined under an inverted microscope (Zeiss) equipped with phase-contrast optics. Indirect immunofluorescence studies were performed on confluent cells grown on glass slides fixed with ice-cold methanol or 4% formaldehyde. Cells were processed for immunofluorescence using anti-Na+/K+-ATPase α-1 monoclonal antibody (Millipore) and anti-zona occludens-1 (anti-ZO-1) polyclonal antibodies (Millipore) and were examined under a Leica confocal microscope.
Western blotting
Cells were grown to confluence, washed in cold PBS and lysed by scraping them off in boiling Laemmli sample buffer containing 20 mM 2-mercaptoethanol. Lysate was boiled for 5 min and viscosity was reduced by sonication using four bursts of 30 s each with 15 s on ice between each sonication step. Lysate was boiled as before and centrifuged at 20000 g for 5 min. The supernatant was run on 10% polyacrylamide gels, transferred to nitrocellulose membranes, blocked in TBS/0.05% Tween 20/5% non-fat dry milk and probed with antibodies diluted in the same solution. The following primary antibodies were used: polyclonal anti-PI5P4Kγ 1:2500 [9], monoclonal anti-α tubulin 1:5000 (Sigma–Aldrich). After washing in TBS/0.05% Tween and incubation with secondary antibody, protein was detected by Super Signal West Femto Maximum Sensitivity Substrate (Thermo Scientific).
QT (quantitative)-PCR
Cellular RNA was extracted with Trizol reagent (Invitrogen) and 1 ng was reverse transcribed with Superscript III and random hexamers (Invitrogen). Quantification of gene expression of the three PI5P4K isoforms was assessed by using TaqMan Gene Expression Assays and TaqMan Gene Expression Master Mix (Life Technologies), using TATA-box-binding protein (TBP) as the reference gene.
RESULTS AND DISCUSSION
NIH-12848 is a specific PI5P4Kγ inhibitor
NIH-12848 (Figure 1) initially emerged as ‘compound 1’ from a kinome profiling study, measuring its selectivity against a panel of 460 kinases (see Supplementary Figure S3 in ref [24]). As can be seen in the supporting information (Supplementary Table S2), NIH-12848 was exquisitely selective for PI5P4Kγ, with an apparent IC50 of 2–3 μM (Supplementary Figure S1). This screen did not include PI5P4Kα (Supplementary Figure S2) and to clarify the actions of NIH-12848 against PI5P4Ks we tested it in PI5P4K assays using PI5P and 32P-γ-ATP as substrates (see Materials and Methods). Under these conditions, PI5P4Kα was not inhibited by 100 μM NIH-12848 (Supplementary Figure S3A) and PI5P4Kβ showed a small but significant stimulation at the same concentration (Supplementary Figure S3B), whereas PI5P4Kγ was inhibited with an apparent IC50 of 3.3 μM (Figure 2), similar to the apparent Kd in the kinome profiling (above).
Structure of NIH-12848
Effect of inhibitor NIH-12848 on PI5P4Kγ activity
Because PI5P4Kγ has a very low catalytic activity [12], these assays use a final enzyme concentration of around 2.3 μM, so it is possible that NIH-12848 is actually more potent and we are, in part, titrating the enzyme protein. We therefore also tested NIH-12848 on nanomolar levels of the PI5P4Kγ+ mutant [12], which has a 300-fold higher activity than wild–type (WT) PI5P4Kγ and we found the IC50 of NIH-12848 to be about 1 μM (Supplementary Figure S3C). Below we show that the PI5P-binding site is the likely site of interaction for NIH-12848 and because the lipid PI5P does not have a precise concentration (not least this depends on its physicochemical structure), the number we deduce here can only be an empirical IC50, not an absolute Ki.
NIH-12848 interacts with the PI5P-binding site on PI5P4Kγ
Most of the mutations introduced into PI5P4Kγ to create PI5P4Kγ+ [12] involve the changing of residues that bind or stabilize ATP to the equivalent residues present in the much more active PI5P4Kα. So the marked contrast in sensitivities to NIH-12848 of PI5P4Kγ+ compared with PI5P4Kα suggested that NIH-12848 could be unusual among kinase inhibitors in that it might not be interacting with the ATP-binding site. This possibility was supported by an inability of NIH-12848 to inhibit the intrinsic ATPase activity of PI5P4Kγ (Figure 2B) and by the observation that including 100 μM ATP in assays using PI5P4Kγ+ (Km for ATP, 2 μM [12]) had only a small effect on the ability of NIH-12848 to inhibit the enzyme near its IC50 concentration: 2 μM NIH-124848 inhibited PI5P4Kγ+ by 40±3.2% with carrier-free (approximately 16 nM) ATP and 64±2.8% with 100 μM ATP; the small shift in NIH-12848 sensitivity is probably an allosteric effect (see below).
To determine directly the interaction site of NIH-12848 with PI5P4Kγ and PI5P4Kγ+, we turned to HDX–MS, a powerful tool for mapping protein conformational changes and binding epitope sites of interaction on proteins [21,25]. There are two peptides in PI5P4Kγ whose rate of HDX is altered significantly in the presence of NIH-12848 (Figures 3B–3D). One peptide spans residues 373–407 (Figures 3C and 3D), part of the enzyme's activation loop, which has been shown to govern the substrate specificity of PI5P4Ks and PI4P5Ks [26,27]. There was also a change in a peptide spanning residues 158–162 (Figures 3C and 3D), which is part of an α-helix that is near the activation loop in PI5P4Kγ (PDB 2GK9), designated α-helix 4 in the original annotated PI5P4Kβ structure [28] (Figures 3B and 3F). Intriguingly, in the PI5P4Kγ+ there was a significant increase in exchange in residues 158–162 compared with WT PI5P4Kγ and a correspondingly larger decrease in exchange upon addition of NIH-12848 (Figure 3E). These data point to the proposed PI5P-binding site of PI5P4Ks [26,27,28] as the likely area of NIH-12848 interaction.
Binding of NIH-12848 to PI5P4Kγ studied by HDX–MS
PI5P4Kγ can be made resistant to NIH-12848
Direct competition experiments (increasing PI5P and looking at the effect that this has on NIH-12848 potency in vitro) would be the most direct route to confirming the above suggestion. However, NIH-12848 is a hydrophobic compound, as is PI5P and it is likely that the two compounds would form mixed micellar structures when together in aqueous buffers, so any such ‘competition’ would be uninterpretable. To seek confirmation for the site of interaction of NIH-12848 we therefore undertook a series of mutational experiments, focusing on the regions of interaction suggested by the HDX–MS data. In the region 373–407 (see above), only Glu378 differs between PI4P5K isoforms and mutating this residue to the PI5P4Kα equivalent histidine made no detectable difference to the activity or NIH-12848 sensitivity of the enzyme (result not shown). In residues 158–162 (see above), Ile159 and Asp161 are both valines in PI5P4Ks α and β and mutating these together in PI5P4Kγ+ caused a small shift in sensitivity to NIH-12848 [inhibition by 10 μM NIH-12848 was 96% ± 1.5 in WT PI5P4Kγ+ and 71% ± 2.6 in the (E378H,I159V,D161V) PI5P4Kγ+ mutant].
Examining this region closely in the structure (Figure 3F) suggested three other residues that might interact with the substrate or inhibitor and which are different between PI5P4K γ compared with α/β. These are Ser164, Asn165 and Glu153, which are respectively asparagine, isoleucine and threonine in PI5P4Kα/β; of these, Glu153 has already been mutated to the PI5P4Kα/β-equivalent threonine in creating PI5P4Kγ+ [12]. We therefore introduced all five mutations of potential interest (Q378H, I159V, D161V, S164N, N165I) into PI5P4Kγ and PI5P4Kγ+ and both these mutant enzymes were completely resistant to NIH-12848 inhibition (Supplementary Figures S4A and S4B).
These data support the HDX–MS data suggesting that NIH-12848 is binding to this area of the PI5P4Kγ protein and they also imply that Ser164 and/or Asn165 are the major contributors to the sensitivity of PI5P4Kγ to NIH-12848. So we mutated only those two residues in PI5P4Kγ to their PI5P4Ks α/β homologues individually or together and found that whereas S164N PI5P4Kγ is as sensitive to NIH-12848 inhibition as WT PI5P4Kγ (Supplementary Figure S4C), N165I PI5P4Kγ is completely insensitive, with 50 μM NIH-12848 having no effect on this mutant (Figure 4). Note that the activity of N165I PI5P4Kγ was 6.7× higher than WT PI5P4Kγ in these assays and Supplementary Figure S4(D) summarizes the activities and sensitivities to NIH12848 of the mutants discussed in the present study.
Effect of inhibitor NIH-12848 on PI5P4Kγ harbouring a single amino acid substitution
Overall, these data show that Asn165 is the residue in PI5P4Kγ that is almost entirely responsible for its sensitivity to NIH-12848. There was no detectable change in the amide exchange rate at Asn165 when NIH-12848 binds to PI5P4Kγ or PI5P4Kγ+ (Figure 3) and the simplest interpretation is that NIH-12848 binds to the activation loop and α-helix 4 to induce a change in the structure in this region and that introducing an isoleucine (as in PI5P4Ks α and β) at residue 165 prevents this structural change from happening, perhaps because of a hydrophobic interaction with the activation loop (Figure 3F). Whatever the molecular mechanism, this residue could, in the future, be mutated in both alleles of the PI5P4Kγ gene in a cell line or a mouse to engender NIH-12848 resistance of the PI5P4Kγ, which would be a powerful chemical biology approach to eliminate completely any potential non-specific cellular effects of NIH-12848. Introducing the complementary mutations into PI5P4Ks α or β, possibly to make them NIH-12848-sensitive, could be an equally informative avenue of investigation.
Effects of NIH-12848 on a kidney cell line
PI5P4Kγ is widely expressed in mammalian tissues, but is particularly high and is indeed the principal PI5P4K isoform in kidney [9]. It is expressed there in epithelial cells, particularly in the thick ascending limb and CCD, so to explore its cellular functions we chose mpkCCD cells, a cell line derived from epithelial cells in the cortical collecting duct of the kidney [22,23]. Western blotting confirmed an easily detectable level of expression of PI5P4Kγ in mpkCCD cells (Figure 6A). These cells grow to confluence and then form gap junctions between them. They then begin to form ‘domes’ in which they show a property of transporting epithelia when cultured in vitro on solid supports. ‘Domes’ are fluid-filled blisters formed between the solid growth surface and the cell layer and their formation is regarded as a sign of differentiated epithelia with active transport processes and with an intact epithelial barrier due to functional cell–cell contacts [22,23]. We found using quantitative PCR (QT-PCR) that while reaching confluence there is a three-fold increase in PI5P4Kγ mRNA, but no significant change in the two other PI5P4K isoforms (Table 1). We thought that PI5P4Kγ might be involved in epithelial cell polarity because in adult mammalian kidney PI5P4Kγ is present in intracellular vesicles with a distribution polarized towards the lumen [9] and in zebra fish (Danio) it is highly expressed in the pro-nephric duct throughout development (ZFIN Database: ZDB-IMAGE-030829-506).
Gene . | PI5P4Kγ . | PI5P4Kα . | PI5P4Kβ . |
---|---|---|---|
Polarized mpkCCD cells | 3.19–3.46 | 0.99–1.01 | 0.51–0.53 |
Gene . | PI5P4Kγ . | PI5P4Kα . | PI5P4Kβ . |
---|---|---|---|
Polarized mpkCCD cells | 3.19–3.46 | 0.99–1.01 | 0.51–0.53 |
The relative expression of the three genes PI5P4Kγ, α and β, with TBP as a reference gene, was determined in a reverse transcription quantitative PCR (RT-qPCR) assay. Values are expressed as fold difference in polarized cells (4 days after reaching confluence when domes are formed) relative to pre-confluent cells (no domes present).
One easily monitored parameter is the redistribution of Na+/K+-ATPase from the cytoplasm to the basolateral plasma membrane that occurs when the cells reach confluence [29]. We explored the effect of 10 μM NIH-12848 (1 μM had similar but smaller effects) and found a complete inhibition of this redistribution, although the cells remained healthy and confluent, with gap junctions formed as revealed by immunocytochemistry of ZO-1 (Figure 5A). From this, we suggest PI5P4Kγ is involved in the progress towards a polarized epithelial phenotype.
Effects of NIH-12848 on mpkCCD cells
Consistent with this possibility, the formation of ‘domes’ (see above) was also markedly inhibited (Figure 5B) by NIH-12848. Moreover, if NIH-12848 was added subsequent to dome formation, the domes disappeared with a detectable effect within 3 h that was maximal by 24 h (Figure 5B), indicating a dynamic relationship between PI5P4Kγ and the development and maintenance of this morphology. The molecular mechanisms underlying this relationship will require much further investigation, but the reported link between PI5P4Kγ and Rho [11], taken together with the well-known involvement of Rho in epithelial cell transport and polarity [30,31], offers one possibility for exploration.
RNAi knockdown of PI5P4Kγ
Our extensive molecular exploration of the interaction of NIH-12848 with PI5P4Kγ (above) emphasizes its remarkable specificity, but nevertheless it is possible that the effects we see are due to an off-target action. To gather some independent evidence that inhibition of PI5P4Kγ underlies the effects of NIH-12848 and thus to begin a validation of this inhibitor as a tool for exploring PI5P4Kγ function, we knocked down PI5P4Kγ in the mpkCCD cells by RNAi. Figure 6(A) and Supplementary Table S3 shows that the knockdown was specific and successful and Figures 6(B) and 6(C) show the effects of PI5P4Kγ knockdown on Na+/K+-ATPase re-localization (Figure 6B) and dome formation (Figure 6C). Within the limits of these analyses, both effects of NIH-12848 are mimicked by this knockdown.
Effect of RNAi Knock-down of PI5P4Kγ or mpkCCD cells
Control experiments knocking down PI5P4Kα and β did not show the same effect on dome formation. PI5P4Kβ knockdown had no detectable effect (Supplementary Figure S5B), whereas knockdown of PI5P4Kα had a pronounced opposite (stimulatory) effect (Supplementary Figures S5A and S4B). We have no immediate explanation for this latter action, which we believe is likely to be independent of PI5P4Kγ activity because knockdown of PI5P4Kγ and PI5P4Kα together showed that PI5P4Kγ causes a similar and internally consistent inhibitory effect on the higher level of dome formation resulting from PI5P4Kα knockdown (Supplementary Figure S5C). Further studies, including using PI5P4Kα inhibitors (e.g. [15]) will be required to clarify this function of PI5P4Kα and its relationship with PI5P4Kγ. We have discussed elsewhere [12] the possibility that PI5P4Kγ may sometimes serve to target the more active PI5P4Kα to a specific cellular location.
Overall, these experiments yield the first functional insight into PI5P4Kγ's physiological role in epithelial cells. Much remains to be explored to clarify exactly what the enzyme is doing in this context, but the very similar effects induced in mpkCCD cells by inhibition with NIH-12848 and knockdown of PI5P4Kγ are a crucial first stage in a proof of principle of the potential usefulness of NIH-12848 in such an exploration. Our detailed mapping and manipulation of the molecular interaction of NIH-12848 with PI5P4Kγ also suggests ways to a wider use of this inhibitor in the exploration of functions of the PI5P4K family.
Abbreviations
- HDX
hydrogen–deuterium exchange
- HDX-MS
hydrogen-deuterium exchange mass spectrometry
- mpkCCD
mouse principal kidney cortical collecting duct
- PI5P
phosphatidylinositol 5-phosphate
- QT-PCR
quantitative PCR
- TBP
TATA-box-binding protein
- TCEP
tris carboxyl ethyl phosphine
- UPLC
ultra performance liquid chromatography
- WT
wild-type
AUTHOR CONTRIBUTION
Jon Clarke engineered and produced recombinant proteins, performed in vitro assays and processed data; Maria-Luisa Giudici performed cell biology experiments and QT-PCR and processed data; John Burke performed the HDX-MS, processed data and interpreted protein structures; Roger Williams processed HDX-MS data and interpreted protein structures; David Maloney and Juan Marugan performed the initial discovery of the PI5P4Kγ–NIH-12848 interaction; Robin Irvine performed some in vitro assays and processed data. All the authors discussed the results, interpreted data and co-wrote the paper.
We are very grateful to Dr A. Vandewalle (INSERM U773, Paris, France) for provision of the mpkCCD cell line and Simon Bulley for his help and advice with the QT-PCR.
FUNDING
This work was supported by the Medical Research Council [grant number RG64071 (to J.H.C.)]; the Biotechnology and Biological Sciences Research Council [grant number RG65394 (to M.-L.G.)]; and the British Heart Foundation [grant number PG11/109/29247 (to J.E.B.)].
References
Author notes
These authors contributed equally to this study.
Present address: Department of Biochemistry and Microbiology, University of Victoria, 270a Petch Hall, Victoria, British Columbia, Canada V8W 2Y2.