Volume 466 (2015), pp. 177–188
An error introduced in the production process resulted in a deleted panel in Figure 6. The error appears in the printed journal; the online version is not affected.
The figure should be as follows:
The role of residues in close proximity to AR-C155858 during the inward-intermediate simulation in inhibitor binding
(A) The position of residues M65, M69, L274 and S278 relative to AR-C155858 is shown after 200 ns simulation in the inward-intermediate conformation of MCT1. (B) The distance between AR-C155858 and residues M65, M69, L274 and S278 during the time course of the simulation. Distances shown are between atoms: M65S to AR-C–HG1; M69CE to AR-C–H121; L274 HG to AR-C–SE1 and S278OG to AR-C–H103. (C). Inhibition of lactate uptake by M65L/M69L, L274P and S278V mutants in response to increasing concentrations of AR-C155858. (D) Plasma membrane expression of the MCT1 mutants shown by immunofluorescence microscopy and Western blot using the C-terminal MCT1 antibody.
(A) The position of residues M65, M69, L274 and S278 relative to AR-C155858 is shown after 200 ns simulation in the inward-intermediate conformation of MCT1. (B) The distance between AR-C155858 and residues M65, M69, L274 and S278 during the time course of the simulation. Distances shown are between atoms: M65S to AR-C–HG1; M69CE to AR-C–H121; L274 HG to AR-C–SE1 and S278OG to AR-C–H103. (C). Inhibition of lactate uptake by M65L/M69L, L274P and S278V mutants in response to increasing concentrations of AR-C155858. (D) Plasma membrane expression of the MCT1 mutants shown by immunofluorescence microscopy and Western blot using the C-terminal MCT1 antibody.
