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Volume 193, Issue 3

March 1981

All Issues

Cover Image

Cover Image
Front Matter
Table of Contents
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ISSN 0264-6021

EISSN 1470-8728

In this Issue

Research Article
Enzymes and Enzyme Kinetics
Peptide and Protein Structure
B.J. Letters
Correction

Research Article

Concanavalin A, a receptor protein with apparently co-operative binding characteristics

E Köttgen; C Bauer; U Ehlerding; W Gerok

Biochem J (1981) 193 (3): 659–662.

Abstract

View Articletitled, Concanavalin A, a receptor protein with apparently co-operative binding characteristics

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Purification and characterization of a lysosomal form and a variant form of β-glucuronidase from the rat basophil leukaemia tumour

L B Schwartz; K F Austen

Biochem J (1981) 193 (3): 663–670.

Abstract

View Articletitled, Purification and characterization of a lysosomal form and a variant form of β-glucuronidase from the rat basophil leukaemia tumour

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Conformational changes in rat liver chromatin after liver regeneration

H Simpkins; L M Thompson; N Waldeck; D S Gross; D Mooney

Biochem J (1981) 193 (3): 671–678.

Abstract

View Articletitled, Conformational changes in rat liver chromatin after liver regeneration

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Nature of the thiamin-binding protein from chicken egg yolk

K Muniyappa; P R Adiga

Biochem J (1981) 193 (3): 679–685.

Abstract

View Articletitled, Nature of the thiamin-binding protein from chicken egg yolk

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A study of the magnetic properties of haem a₃ in cytochrome c oxidase by using magnetic-circular-dichroism spectroscopy

A J Thomson; M K Johnson; C Greenwood; P E Gooding

Biochem J (1981) 193 (3): 687–697.

Abstract

View Articletitled, A study of the magnetic properties of haem a3 in cytochrome c oxidase by using magnetic-circular-dichroism spectroscopy

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Characterization of the partially reduced cyanide-inhibited derivative of cytochrome c oxidase by optical, electron-paramagnetic-resonance and magnetic-circular-dichroism spectroscopy

M K Johnson; D G Eglinton; P E Gooding; C Greenwood; A J Thomson

Biochem J (1981) 193 (3): 699–708.

Abstract

View Articletitled, Characterization of the partially reduced cyanide-inhibited derivative of cytochrome c oxidase by optical, electron-paramagnetic-resonance and magnetic-circular-dichroism spectroscopy

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Properties of rat renal phosphate-dependent glutaminase coupled to Sepharose. Evidence that dimerization is essential for activation

R F Morehouse; N P Curthoys

Biochem J (1981) 193 (3): 709–716.

Abstract

View Articletitled, Properties of rat renal phosphate-dependent glutaminase coupled to Sepharose. Evidence that dimerization is essential for activation

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The influence of pH on the equilibrium distribution of iron between the metal-binding sites of human transferrin

N D Chasteen; J Williams

Biochem J (1981) 193 (3): 717–727.

Abstract

View Articletitled, The influence of pH on the equilibrium distribution of iron between the metal-binding sites of human transferrin

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Influence of the α-, β- and γ-subunits of the energy-transducing adenosine triphosphates from Micrococcus lysodeikticus in the immunochemical properties of the protein and in their reconstitution studied by a radioimmunoassay method

V Larraga; F Mollinedo; N Rubio; E Muñoz

Biochem J (1981) 193 (3): 729–735.

Abstract

View Articletitled, Influence of the α-, β- and γ-subunits of the energy-transducing adenosine triphosphates from Micrococcus lysodeikticus in the immunochemical properties of the protein and in their reconstitution studied by a radioimmunoassay method

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Cleavage by trypsin and by the proteinase from Armillaria mellea at ɛ-N-formyl-lysine residues

F P Barry; S Doonan; C A Ross

Biochem J (1981) 193 (3): 737–742.

Abstract

View Articletitled, Cleavage by trypsin and by the proteinase from Armillaria mellea at ɛ-N-formyl-lysine residues

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Subcellular localization of low-molecular-weight RNA components in rat liver

S Frederiksen; H Flodgard; P Hellung-Larsen

Biochem J (1981) 193 (3): 743–748.

Abstract

View Articletitled, Subcellular localization of low-molecular-weight RNA components in rat liver

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Isolation and characterization of pepsin fragments of laminin from human placental and renal basement membranes

L Risteli; R Timpl

Biochem J (1981) 193 (3): 749–755.

Abstract

View Articletitled, Isolation and characterization of pepsin fragments of laminin from human placental and renal basement membranes

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Photolabelling of membrane proteins with photoactive phospholipids

R Bisson; C Montecucco

Biochem J (1981) 193 (3): 757–763.

Abstract

View Articletitled, Photolabelling of membrane proteins with photoactive phospholipids

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Fractionation of cell-wall preparations from grass leaves by centrifuging in non-aqueous density gradients

A H Gordon; J S Bacon

Biochem J (1981) 193 (3): 765–771.

Abstract

View Articletitled, Fractionation of cell-wall preparations from grass leaves by centrifuging in non-aqueous density gradients

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The stability and aggregation properties of human liver acid β-d-galactosidase

C M Heyworth; E F Neumann; C H Wynn

Biochem J (1981) 193 (3): 773–779.

Abstract

View Articletitled, The stability and aggregation properties of human liver acid β-d-galactosidase

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The extraction from maize (Zea mays) root cells of membrane-bound protein with Ca²⁺-dependent ATPase activity and its possible role in membrane fusion in vitro

E A H Baydoun; D H Northcote

Biochem J (1981) 193 (3): 781–792.

Abstract

View Articletitled, The extraction from maize (Zea mays) root cells of membrane-bound protein with Ca2+-dependent ATPase activity and its possible role in membrane fusion in vitro

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Radiochemical methods for the assay of phosphorylase in the direction of glycogenolysis

G Gevers; W Stalmans

Biochem J (1981) 193 (3): 793–798.

Abstract

View Articletitled, Radiochemical methods for the assay of phosphorylase in the direction of glycogenolysis

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Studies on the mechanism of action of 6-mercaptopurine. Interaction with copper and xanthine oxidase

U Kela; R Vijayvargiya

Biochem J (1981) 193 (3): 799–803.

Abstract

View Articletitled, Studies on the mechanism of action of 6-mercaptopurine. Interaction with copper and xanthine oxidase

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Identification of the apparently essential lysine residues in phospholipase C (Bacillus cereus)

B J Myrnes; C Little

Biochem J (1981) 193 (3): 805–809.

Abstract

View Articletitled, Identification of the apparently essential lysine residues in phospholipase C (Bacillus cereus)

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Degradation of keratan sulphate by β-N-acetylhexosaminidases A and B

T Ludolph; E Paschke; J Glössl; H Kresse

Biochem J (1981) 193 (3): 811–818.

Abstract

View Articletitled, Degradation of keratan sulphate by β-N-acetylhexosaminidases A and B

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Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe

K Brocklehurst; J P G Malthouse

Biochem J (1981) 193 (3): 819–823.

Abstract

View Articletitled, Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe

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Chemical cross-linking of a dimeric protein on a modified lectin matrix. A general probe for the chemical topology of oligomeric glycoproteins

S Pillai

Biochem J (1981) 193 (3): 825–828.

Abstract

View Articletitled, Chemical cross-linking of a dimeric protein on a modified lectin matrix. A general probe for the chemical topology of oligomeric glycoproteins

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The effects of bivalent cations on ribulose bisphosphate carboxylase/oxygenase

J T Christeller

Biochem J (1981) 193 (3): 839–844.

Abstract

View Articletitled, The effects of bivalent cations on ribulose bisphosphate carboxylase/oxygenase

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Fatty-acid desaturation and microsomal lipid fatty-acid composition in experimental hyperthyroidism

F H Faas; W J Carter

Biochem J (1981) 193 (3): 845–852.

Abstract

View Articletitled, Fatty-acid desaturation and microsomal lipid fatty-acid composition in experimental hyperthyroidism

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Inactivation of rat muscle 5′-adenylate aminohydrolase by tyrosine nitration with tetranitromethane

M Ranieri-Raggi; C Bergamini; U Montali; A Raggi

Biochem J (1981) 193 (3): 853–859.

Abstract

View Articletitled, Inactivation of rat muscle 5′-adenylate aminohydrolase by tyrosine nitration with tetranitromethane

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Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12

R H Jackson; A Cornish-Bowden; J A Cole

Biochem J (1981) 193 (3): 861–867.

Abstract

View Articletitled, Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12

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Studies in vitro on the involvement of O-sulphate esters in the formation of O-methylated 3,4-dihydroxybenzoic acid by rat liver

E J M Pennings; G M J Van Kempen

Biochem J (1981) 193 (3): 869–874.

Abstract

View Articletitled, Studies in vitro on the involvement of O-sulphate esters in the formation of O-methylated 3,4-dihydroxybenzoic acid by rat liver

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Isolation and characterization of calmodulin from an insulin-secreting tumour

J C Hutton; E J Penn; P Jackson; C N Hales

Biochem J (1981) 193 (3): 875–885.

Abstract

View Articletitled, Isolation and characterization of calmodulin from an insulin-secreting tumour

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Immunoelectrophoretic studies on human small-intestinal brush-border proteins

H Skovbjerg

Biochem J (1981) 193 (3): 887–890.

Abstract

View Articletitled, Immunoelectrophoretic studies on human small-intestinal brush-border proteins

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Actin from pig and rat uterus

J S Elce; A S U Elbrecht; M U Middlestadt; E J McIntyre; P J Anderson

Biochem J (1981) 193 (3): 891–898.

Abstract

View Articletitled, Actin from pig and rat uterus

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Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus)

H S Kim; N Tamiya

Biochem J (1981) 193 (3): 899–906.

Abstract

View Articletitled, Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus)

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Platelet-derived growth factor. Isolation by a large-scale procedure and analysis of subunit composition

C H Heldin; B Westermark; A Wasteson

Biochem J (1981) 193 (3): 907–913.

Abstract

View Articletitled, Platelet-derived growth factor. Isolation by a large-scale procedure and analysis of subunit composition

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Desmosines in human urine. Amounts in early development and in Marfan's syndrome

Z Gunja-Smith; R J Boucek

Biochem J (1981) 193 (3): 915–918.

Abstract

View Articletitled, Desmosines in human urine. Amounts in early development and in Marfan's syndrome

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Tritiation of α-bungarotoxin with N-succinimidyl [2,3-³H]propionate. A useful reagent for labelling proteins

J O Dolly; E A V Nockles; M M S Lo; E A Barnard

Biochem J (1981) 193 (3): 919–923.

Abstract

View Articletitled, Tritiation of α-bungarotoxin with N-succinimidyl [2,3-3H]propionate. A useful reagent for labelling proteins

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Occupancy of sites of phosphorylation in inactive rat heart pyruvate dehydrogenase phosphate in vivo

G J Sale; P J Randle

Biochem J (1981) 193 (3): 935–946.

Abstract

View Articletitled, Occupancy of sites of phosphorylation in inactive rat heart pyruvate dehydrogenase phosphate in vivo

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Size characteristics of human leucocyte interferon under reducing and non-reducing conditions

I A Braude; L S Lin; W E Stewart, 2nd

Biochem J (1981) 193 (3): 947–951.

Abstract

View Articletitled, Size characteristics of human leucocyte interferon under reducing and non-reducing conditions

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Amino acid sequence and oligosaccharide distribution of the haemagglutinin from an early Hong Kong influenza virus variant A/Aichi/2/68 (X-31)

C W Ward; T A Dopheide

Biochem J (1981) 193 (3): 953–962.

Abstract

View Articletitled, Amino acid sequence and oligosaccharide distribution of the haemagglutinin from an early Hong Kong influenza virus variant A/Aichi/2/68 (X-31)

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The separation of functionally distinct forms of the third component of human complement (C3)

C Parkes; R G DiScipio; M A Kerr; R Prohaska

Biochem J (1981) 193 (3): 963–970.

Abstract

View Articletitled, The separation of functionally distinct forms of the third component of human complement (C3)

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Nitrogenase of Klebsiella pneumoniae. Hydrazine is a product of azide reduction

M J Dilworth; R N F Thorneley

Biochem J (1981) 193 (3): 971–983.

Abstract

View Articletitled, Nitrogenase of Klebsiella pneumoniae. Hydrazine is a product of azide reduction

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Differential inhibition of rat liver DNA polymerases in vitro by direct-acting carcinogens and the protective effect of a thiol reducing agent

J Y Chan; F F Becker

Biochem J (1981) 193 (3): 985–990.

Abstract

View Articletitled, Differential inhibition of rat liver DNA polymerases in vitro by direct-acting carcinogens and the protective effect of a thiol reducing agent

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The generation of non-linear solute gradients for chromatography by using only simple apparatus

F Solano-Muñoz; W G Bardsley

Biochem J (1981) 193 (3): 991–996.

Abstract

View Articletitled, The generation of non-linear solute gradients for chromatography by using only simple apparatus

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DNA polymerase of a basidiomycete fungus, Coprinus cinereus

D Lowe; D Lewis

Biochem J (1981) 193 (3): 997–1004.

Abstract

View Articletitled, DNA polymerase of a basidiomycete fungus, Coprinus cinereus

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Fitting of enzyme kinetic data without prior knowledge of weights

A Cornish-Bowden; L Endrenyi

Biochem J (1981) 193 (3): 1005–1008.

Abstract

View Articletitled, Fitting of enzyme kinetic data without prior knowledge of weights

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An easy method for the determination of initial rates

S G Waley

Biochem J (1981) 193 (3): 1009–1012.

Abstract

View Articletitled, An easy method for the determination of initial rates

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A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels

G D Jones; M T Wilson; V M Darley-Usmar

Biochem J (1981) 193 (3): 1013–1015.

Abstract

View Articletitled, A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels

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Formation of a double salt of phosphatidylcholine and zinc chloride

K I von Glós; J C Boursnell

Biochem J (1981) 193 (3): 1017–1019.

Abstract

View Articletitled, Formation of a double salt of phosphatidylcholine and zinc chloride

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N.m.r. and e.p.r. characterization of [4-carboxy-2,6-dinitrophenyl-lysine]cytochromes c

K E Falk; P A Jovall; J Angström

Biochem J (1981) 193 (3): 1021–1024.

Abstract

View Articletitled, N.m.r. and e.p.r. characterization of [4-carboxy-2,6-dinitrophenyl-lysine]cytochromes c

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Neutral glycolipids and gangliosides of concanavalin A-selected SV3T3 revertant cells and of normal and SV40-transformed Balb/c 3T3 cells

G Mugnai; R Coppini; D Tombaccini; A Fallani; S Ruggieri

Biochem J (1981) 193 (3): 1025–1028.

Abstract

View Articletitled, Neutral glycolipids and gangliosides of concanavalin A-selected SV3T3 revertant cells and of normal and SV40-transformed Balb/c 3T3 cells

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Kinetics of the photochemical interconversion among geometric photoisomers of bilirubin

K Isobe; S Onishi

Biochem J (1981) 193 (3): 1029–1031.

Abstract

View Articletitled, Kinetics of the photochemical interconversion among geometric photoisomers of bilirubin

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Enzymes and Enzyme Kinetics

Cyclic AMP-independent casein/glycogen synthase kinases from pig polymorphonuclear leucocytes

J. Manuel Pena; Roser Cussó; Emilio Itarte

Biochem J (1981) 193 (3): 829–837.

Abstract

View Articletitled, Cyclic AMP-independent casein/glycogen synthase kinases from pig polymorphonuclear leucocytes

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Peptide and Protein Structure

Influence of myosin heavy chains on the Ca²⁺-binding properties of light chain, LC₂

S. Srivastava; A. Muhlrad; J. Wikman-Coffelt

Biochem J (1981) 193 (3): 925–934.

Abstract

View Articletitled, Influence of myosin heavy chains on the Ca2+-binding properties of light chain, LC2

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B.J. Letters

Estimation of K_m values of enzymes requiring molecular O₂ as a substrate

Robin H. C. Strang

Biochem J (1981) 193 (3): 1033–1034.

View Articletitled, Estimation of Km values of enzymes requiring molecular O2 as a substrate

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The structure of acetyldihydrolipoic acid

Geoffrey Hale; Henry B. F. Dixon

Biochem J (1981) 193 (3): 1034–1035.

View Articletitled, The structure of acetyldihydrolipoic acid

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Correction

The photoinduced isomerization of bilirubin in cationic detergent solutions

Biochem J (1981) 193 (3): 1037.

View Articletitled, The photoinduced isomerization of bilirubin in cationic detergent solutions

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B.J. Letters

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