Issues

The cover of this edition of Biochemical Journal features images of epidermal cells from Nicotiana benthamiana leaves. The top two rows feature representative confocal microscopy images of epidermal cells transiently expressing 35::IRT1-mCitrine alongside a range of mutants following control treatment or non-iron metal excess treatment. Scale bars indicate 20 µm. The bottom two rows feature a TriFC assay with CIPK23 fused to an mCitC fragment and ALFA tag-bearing IRT1 variants co-expressed with ALFA-NB C-terminally fused to the mCitN fragment. CIPK24, which does not interact with IRT1, is used as negative control. Scale bars indicate 10 µm. The collective observations implicate residue D173 in Zn2+ and Mn2+ sensing and proper IRT1 degradation when plants are experiencing non-iron metal stress. Read more from Vert and colleagues in ‘Metal-sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1’ at: https://doi.org/10.1042/BCJ20240685