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Articles

Loss of AMP-activated protein kinase α2 subunit in mouse β-cells impairs glucose-stimulated insulin secretion and inhibits their sensitivity to hypoglycaemia

Craig Beall, Kaisa Piipari, Hind Al-Qassab, Mark A. Smith, Nadeene Parker, David Carling, Benoit Viollet, Dominic J. Withers, Michael L. J. Ashford
Journal: Biochemical Journal
Biochem J (2010) 429 (2): 323–333.
https://doi.org/10.1042/BJ20100231
Published: 28 June 2010
View article titled, Loss of AMP-activated protein kinase α2 subunit in mouse β-cells impairs glucose-stimulated insulin secretion and inhibits their sensitivity to hypoglycaemia
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Includes: Supplementary data
Articles

The role of OXA-1 β-lactamase Asp 66 in the stabilization of the active-site carbamate group and in substrate turnover

David A. Leonard, Andrea M. Hujer, Brian A. Smith, Kyle D. Schneider, Christopher R. Bethel, Kristine M. Hujer, Robert A. Bonomo
Journal: Biochemical Journal
Biochem J (2008) 410 (3): 455–462.
https://doi.org/10.1042/BJ20070573
Published: 27 February 2008
View article titled, The role of OXA-1 β-lactamase Asp 66 in the stabilization of the active-site carbamate group and in substrate turnover
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Articles

Anomalous scattering analysis of Agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site

Colin J. Jackson, Paul D. Carr, Hye-Kyung Kim, Jian-Wei Liu, Paul Herrald, Nataša Mitić, Gerhard Schenk, Clyde A. Smith, David L. Ollis
Journal: Biochemical Journal
Biochem J (2006) 397 (3): 501–508.
https://doi.org/10.1042/BJ20060276
Published: 13 July 2006
View article titled, Anomalous scattering analysis of Agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site
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Articles

Mammalian class Sigma glutathione S-transferases: catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases

Ian R. JOWSEY, Anne M. THOMSON, Jack U. FLANAGAN, Paul R. MURDOCK, Gary B. T. MOORE, David J. MEYER, Gregory J. MURPHY, Stephen A. SMITH, John D. HAYES
Journal: Biochemical Journal
Biochem J (2001) 359 (3): 507–516.
https://doi.org/10.1042/bj3590507
Published: 25 October 2001
View article titled, Mammalian class Sigma glutathione S-transferases: catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases
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Articles

The effect of Mg2+ on cardiac muscle function: is CaATP the substrate for priming myofibril cross-bridge formation and Ca2+ reuptake by the sarcoplasmic reticulum?

Gerry A. SMITH, Jamie I. VANDENBERG, Nicholas S. FREESTONE, Henry B. F. DIXON
Journal: Biochemical Journal
Biochem J (2001) 354 (3): 539–551.
https://doi.org/10.1042/bj3540539
Published: 08 March 2001
View article titled, The effect of Mg2+ on cardiac muscle function: is CaATP the substrate for priming myofibril cross-bridge formation and Ca2+ reuptake by the sarcoplasmic reticulum?
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Articles

Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleft

Helen S. TOOGOOD, Clyde A. SMITH, Edward N. BAKER, Roy M. DANIEL
Journal: Biochemical Journal
Biochem J (2000) 350 (1): 321–328.
https://doi.org/10.1042/bj3500321
Published: 09 August 2000
View article titled, Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleft
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Articles

Estimation of systolic and diastolic free intracellular Ca2+ by titration of Ca2+ buffering in the ferret heart

Heide L. KIRSCHENLOHR, Andrew A. GRACE, Jamie I. VANDENBERG, James C. METCALFE, Gerry A. SMITH
Journal: Biochemical Journal
Biochem J (2000) 346 (2): 385–391.
https://doi.org/10.1042/bj3460385
Published: 22 February 2000
View article titled, Estimation of systolic and diastolic free intracellular Ca2+ by titration of Ca2+ buffering in the ferret heart
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Articles

Ca2+ buffering in the heart: Ca2+ binding to and activation of cardiac myofibrils

Gerry A. SMITH, Henry B. F. DIXON, Heide L. KIRSCHENLOHR, Andrew A. GRACE, James C. METCALFE, Jamie I. VANDENBERG
Journal: Biochemical Journal
Biochem J (2000) 346 (2): 393–402.
https://doi.org/10.1042/bj3460393
Published: 22 February 2000
View article titled, Ca2+ buffering in the heart: Ca2+ binding to and activation of cardiac myofibrils
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Articles

Demonstration of specific insulin binding to cytosolic proteins in H35 hepatoma cells, rat liver and skeletal muscle

S Harada, R M Smith, J A Smith, N Shah, L Jarett
Journal: Biochemical Journal
Biochem J (1995) 306 (1): 21–28.
https://doi.org/10.1042/bj3060021
Published: 15 February 1995
View article titled, Demonstration of specific insulin binding to cytosolic proteins in H35 hepatoma cells, rat liver and skeletal muscle
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Articles

Expression of a biologically active plant cytochrome b 5 in Escherichia coli

M A Smith, J A Napier, S Stymne, A S Tatham, P R Shewry, A K Stobart
Journal: Biochemical Journal
Biochem J (1994) 303 (1): 73–79.
https://doi.org/10.1042/bj3030073
Published: 01 October 1994
View article titled, Expression of a biologically active plant cytochrome b 5 in Escherichia coli
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Articles

The synthetic substrate succinyl(carbadethia)-CoA generates cob(II)alamin on adenosylcobalamin-dependent methylmalonyl-CoA mutase

N H Keep, G A Smith, M C W Evans, G P Diakun, P F Leadlay
Journal: Biochemical Journal
Biochem J (1993) 295 (2): 387–392.
https://doi.org/10.1042/bj2950387
Published: 15 October 1993
View article titled, The synthetic substrate succinyl(carbadethia)-CoA generates cob(II)alamin on adenosylcobalamin-dependent methylmalonyl-CoA mutase
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Articles

Substrate-dependent changes in mitochondrial function, intracellular free calcium concentration and membrane channels in pancreatic β -cells

M R Duchen, P A Smith, F M Ashcroft
Journal: Biochemical Journal
Biochem J (1993) 294 (1): 35–42.
https://doi.org/10.1042/bj2940035
Published: 15 August 1993
View article titled, Substrate-dependent changes in mitochondrial function, intracellular free calcium concentration and membrane channels in pancreatic β -cells
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Articles

Calcium measurements with a new high-affinity n.m.r. indicator in the isolated perfused heart

H L Kirschenlohr, A A Grace, S D Clarke, Y Shachar-Hill, J C Metcalfe, P G Morris, G A Smith
Journal: Biochemical Journal
Biochem J (1993) 293 (2): 407–411.
https://doi.org/10.1042/bj2930407
Published: 15 July 1993
View article titled, Calcium measurements with a new high-affinity n.m.r. indicator in the isolated perfused heart
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Articles

Binding of a substrate analogue can induce co-operative structure in the plasmin serine-proteinase domain

A J Teuten, A Cooper, R A Smith, C M Dobson
Journal: Biochemical Journal
Biochem J (1993) 293 (2): 567–572.
https://doi.org/10.1042/bj2930567
Published: 15 July 1993
View article titled, Binding of a substrate analogue can induce co-operative structure in the plasmin serine-proteinase domain
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Articles

Effects of fluorinated inositols on the proliferation of Swiss 3T3 fibroblasts

S C Cosulich, J Offer, G A Smith, R Hesketh, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1993) 292 (3): 719–724.
https://doi.org/10.1042/bj2920719
Published: 15 June 1993
View article titled, Effects of fluorinated inositols on the proliferation of Swiss 3T3 fibroblasts
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Articles

Haem and non-haem iron sites in Escherichia coli bacterioferritin: spectroscopic and model building studies

M R Cheesman, N E le Brun, F H A Kadir, A J Thomson, G R Moore, S C Andrews, J R Guest, P M Harrison, J M A Smith, S J Yewdall
Journal: Biochemical Journal
Biochem J (1993) 292 (1): 47–56.
https://doi.org/10.1042/bj2920047
Published: 15 May 1993
View article titled, Haem and non-haem iron sites in Escherichia coli bacterioferritin: spectroscopic and model building studies
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Articles

The uptake of 3 H-labelled monodeoxyfluoro- myo -inositols into thymocytes and their incorporation into phospholipid in permeabilized cells

J Offer, J C Metcalfe, G A Smith
Journal: Biochemical Journal
Biochem J (1993) 291 (2): 553–560.
https://doi.org/10.1042/bj2910553
Published: 15 April 1993
View article titled, The uptake of 3 H-labelled monodeoxyfluoro- myo -inositols into thymocytes and their incorporation into phospholipid in permeabilized cells
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Articles

Comparative behaviour of calpain and cathepsin B toward peptidyl acyloxymethyl ketones, sulphonium methyl ketones and other potential inhibitors of cysteine proteinases

D H Pliura, B J Bonaventura, R A Smith, P J Coles, A Krantz
Journal: Biochemical Journal
Biochem J (1992) 288 (3): 759–762.
https://doi.org/10.1042/bj2880759
Published: 15 December 1992
View article titled, Comparative behaviour of calpain and cathepsin B toward peptidyl acyloxymethyl ketones, sulphonium methyl ketones and other potential inhibitors of cysteine proteinases
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Articles

Evidence for cytochrome b 5 as an electron donor in ricinoleic acid biosynthesis in microsomal preparations from developing castor bean ( Ricinus communis L.)

M A Smith, L Jonsson, S Stymne, K Stobart
Journal: Biochemical Journal
Biochem J (1992) 287 (1): 141–144.
https://doi.org/10.1042/bj2870141
Published: 01 October 1992
View article titled, Evidence for cytochrome b 5 as an electron donor in ricinoleic acid biosynthesis in microsomal preparations from developing castor bean ( Ricinus communis L.)
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Articles

Ricinoleic acid biosynthesis and triacylglycerol assembly in microsomal preparations from developing castor-bean ( Ricinus communis ) endosperm

M Bafor, M A Smith, L Jonsson, K Stobart, S Stymne
Journal: Biochemical Journal
Biochem J (1991) 280 (2): 507–514.
https://doi.org/10.1042/bj2800507
Published: 01 December 1991
View article titled, Ricinoleic acid biosynthesis and triacylglycerol assembly in microsomal preparations from developing castor-bean ( Ricinus communis ) endosperm
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Articles

Substrate and pH effects on glutamine synthesis in rat liver. Consequences for acid-base regulation

M K Almond, A Smith, R D Cohen, R A Iles, G Flynn
Journal: Biochemical Journal
Biochem J (1991) 278 (3): 709–714.
https://doi.org/10.1042/bj2780709
Published: 15 September 1991
View article titled, Substrate and pH effects on glutamine synthesis in rat liver. Consequences for acid-base regulation
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Articles

The origin of multiply sigmoid curves of pH-dependence. The partitioning of groups among titration p K values

H B F Dixon, S D Clarke, G A Smith, T K Carne
Journal: Biochemical Journal
Biochem J (1991) 278 (1): 279–284.
https://doi.org/10.1042/bj2780279
Published: 15 August 1991
View article titled, The origin of multiply sigmoid curves of pH-dependence. The partitioning of groups among titration p K values
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Articles

d-Xylose (d-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Purification and properties

C A Smith, M Rangarajan, B S Hartley
Journal: Biochemical Journal
Biochem J (1991) 277 (1): 255–261.
https://doi.org/10.1042/bj2770255
Published: 01 July 1991
View article titled, d-Xylose (d-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Purification and properties
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Articles

The murine haemopexin receptor. Evidence that the haemopexin-binding site resides on a 20 kDa subunit and that receptor recycling is regulated by protein kinase C

A Smith, S M Farooqui, W T Morgan
Journal: Biochemical Journal
Biochem J (1991) 276 (2): 417–425.
https://doi.org/10.1042/bj2760417
Published: 01 June 1991
View article titled, The murine haemopexin receptor. Evidence that the haemopexin-binding site resides on a 20 kDa subunit and that receptor recycling is regulated by protein kinase C
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Articles

An acetylation method for the quantification of membrane lipids, including phospholipids, polyphosphoinositides and cholesterol

J M Stein, G A Smith, J P Luzio
Journal: Biochemical Journal
Biochem J (1991) 274 (2): 375–379.
https://doi.org/10.1042/bj2740375
Published: 01 March 1991
View article titled, An acetylation method for the quantification of membrane lipids, including phospholipids, polyphosphoinositides and cholesterol
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Articles

Electron-transport components of the 1-acyl-2-oleoyl- sn -glycero-3-phosphocholine Δ 12 -desaturase (Δ 12 -desaturase) in microsomal preparations from developing safflower ( Carthamus tinctorius L.) cotyledons

M A Smith, A R Cross, O T G Jones, W T Griffiths, S Stymne, K Stobart
Journal: Biochemical Journal
Biochem J (1990) 272 (1): 23–29.
https://doi.org/10.1042/bj2720023
Published: 15 November 1990
View article titled, Electron-transport components of the 1-acyl-2-oleoyl- sn -glycero-3-phosphocholine Δ 12 -desaturase (Δ 12 -desaturase) in microsomal preparations from developing safflower ( Carthamus tinctorius L.) cotyledons
Open the PDF for Electron-transport components of the 1-acyl-2-oleoyl- sn -glycero-3-phosphocholine Δ 12 -desaturase (Δ 12 -desaturase) in microsomal preparations from developing safflower ( Carthamus tinctorius L.) cotyledons in another window
Articles

Identification and immunochemical characterization of the human erythrocyte membrane glycoproteins that carry the Xg a antigen

R Herron, G A Smith
Journal: Biochemical Journal
Biochem J (1989) 262 (1): 369–371.
https://doi.org/10.1042/bj2620369
Published: 15 August 1989
View article titled, Identification and immunochemical characterization of the human erythrocyte membrane glycoproteins that carry the Xg a antigen
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Articles

Expression of the haemopexin-transport system in cultured mouse hepatoma cells. Links between haemopexin and iron metabolism

A Smith, B E Ledford
Journal: Biochemical Journal
Biochem J (1988) 256 (3): 941–950.
https://doi.org/10.1042/bj2560941
Published: 15 December 1988
View article titled, Expression of the haemopexin-transport system in cultured mouse hepatoma cells. Links between haemopexin and iron metabolism
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Articles

Antioxidant protection by haemopexin of haem-stimulated lipid peroxidation

J M Gutteridge, A Smith
Journal: Biochemical Journal
Biochem J (1988) 256 (3): 861–865.
https://doi.org/10.1042/bj2560861
Published: 15 December 1988
View article titled, Antioxidant protection by haemopexin of haem-stimulated lipid peroxidation
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Articles

Design and properties of a fluorescent indicator of intracellular free Na + concentration

G A Smith, T R Hesketh, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1988) 250 (1): 227–232.
https://doi.org/10.1042/bj2500227
Published: 15 February 1988
View article titled, Design and properties of a fluorescent indicator of intracellular free Na + concentration
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Articles

The interaction of streptokinase.plasminogen activator complex, tissue-type plasminogen activator, urokinase and their acylated derivatives with fibrin and cyanogen bromide digest of fibrinogen. Relationship to fibrinolytic potency in vitro

R Cassels, R Fears, R A Smith
Journal: Biochemical Journal
Biochem J (1987) 247 (2): 395–400.
https://doi.org/10.1042/bj2470395
Published: 15 October 1987
View article titled, The interaction of streptokinase.plasminogen activator complex, tissue-type plasminogen activator, urokinase and their acylated derivatives with fibrin and cyanogen bromide digest of fibrinogen. Relationship to fibrinolytic potency in vitro
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Articles

Diurnal variation in the fraction of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the active form in the mammary gland of the lactating rat

R A Smith, B Middleton, D W West
Journal: Biochemical Journal
Biochem J (1986) 239 (2): 285–293.
https://doi.org/10.1042/bj2390285
Published: 15 October 1986
View article titled, Diurnal variation in the fraction of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the active form in the mammary gland of the lactating rat
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Articles

An active-site titrant for human tissue-type plasminogen activator

R A Smith
Journal: Biochemical Journal
Biochem J (1986) 239 (2): 477–479.
https://doi.org/10.1042/bj2390477
Published: 15 October 1986
View article titled, An active-site titrant for human tissue-type plasminogen activator
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Articles

Quantification in vivo of the effects of insulin on glucose utilization in individual tissues of warm- and cold-acclimated rats

S A Smith, P Young, M A Cawthorne
Journal: Biochemical Journal
Biochem J (1986) 237 (3): 789–795.
https://doi.org/10.1042/bj2370789
Published: 01 August 1986
View article titled, Quantification in vivo of the effects of insulin on glucose utilization in individual tissues of warm- and cold-acclimated rats
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Articles

Dicyclohexylamine is not an inhibitor of spermidine synthase

K W Batchelor, R A Smith, N S Watson
Journal: Biochemical Journal
Biochem J (1986) 233 (1): 307–308.
https://doi.org/10.1042/bj2330307
Published: 01 January 1986
View article titled, Dicyclohexylamine is not an inhibitor of spermidine synthase
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Articles

Adenosine effects on glucose oxidation of adipocytes isolated from streptozotocin-diabetic rats

E H Wong, J A Smith, L Jarett
Journal: Biochemical Journal
Biochem J (1985) 232 (1): 301–304.
https://doi.org/10.1042/bj2320301
Published: 15 November 1985
View article titled, Adenosine effects on glucose oxidation of adipocytes isolated from streptozotocin-diabetic rats
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Articles

Intracellular distribution of haem after uptake by different receptors. Haem-haemopexin and haem-asialo-haemopexin

A Smith
Journal: Biochemical Journal
Biochem J (1985) 231 (3): 663–669.
https://doi.org/10.1042/bj2310663
Published: 01 November 1985
View article titled, Intracellular distribution of haem after uptake by different receptors. Haem-haemopexin and haem-asialo-haemopexin
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Articles

Interactions with glutathione S -transferases of porphyrins used in photodynamic therapy and naturally occurring porphyrins

A Smith, I Nuiry, Y C Awasthi
Journal: Biochemical Journal
Biochem J (1985) 229 (3): 823–831.
https://doi.org/10.1042/bj2290823
Published: 01 August 1985
View article titled, Interactions with glutathione S -transferases of porphyrins used in photodynamic therapy and naturally occurring porphyrins
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Articles

Differential interaction of porphyrins used in photoradiation therapy with ferrochelatase

H A Dailey, A Smith
Journal: Biochemical Journal
Biochem J (1984) 223 (2): 441–445.
https://doi.org/10.1042/bj2230441
Published: 15 October 1984
View article titled, Differential interaction of porphyrins used in photoradiation therapy with ferrochelatase
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Articles

Calcium signals and phospholipid methylation in eukaryotic cells

J P Moore, A Johannsson, T R Hesketh, G A Smith, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1984) 221 (3): 675–684.
https://doi.org/10.1042/bj2210675
Published: 01 August 1984
View article titled, Calcium signals and phospholipid methylation in eukaryotic cells
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Articles

Defective activation by glucose of hepatic glycogen synthesis in the obese hyperglycaemic mouse

S A Smith, M A Cawthorne, A L Levy, D L Simson
Journal: Biochemical Journal
Biochem J (1983) 216 (2): 491–494.
https://doi.org/10.1042/bj2160491
Published: 15 November 1983
View article titled, Defective activation by glucose of hepatic glycogen synthesis in the obese hyperglycaemic mouse
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Articles

Large effects of preparative techniques on lymphocyte cyclic AMP content

J P Moore, G A Smith, T R Hesketh, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1983) 216 (1): 207–213.
https://doi.org/10.1042/bj2160207
Published: 15 October 1983
View article titled, Large effects of preparative techniques on lymphocyte cyclic AMP content
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Articles

Haematoporphyrin and OO '-diacetylhaematoporphyrin binding by serum and cellular proteins. Implications for the clearance of these photochemotherapeutic agents by cells

A Smith, T Neuschatz
Journal: Biochemical Journal
Biochem J (1983) 214 (2): 503–509.
https://doi.org/10.1042/bj2140503
Published: 15 August 1983
View article titled, Haematoporphyrin and OO '-diacetylhaematoporphyrin binding by serum and cellular proteins. Implications for the clearance of these photochemotherapeutic agents by cells
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Articles

Duration of the calcium signal in the mitogenic stimulation of thymocytes

T R Hesketh, S Bavetta, G A Smith, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1983) 214 (2): 575–579.
https://doi.org/10.1042/bj2140575
Published: 15 August 1983
View article titled, Duration of the calcium signal in the mitogenic stimulation of thymocytes
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Articles

Limits to the early increase in free cytoplasmic calcium concentration during the mitogenic stimulation of lymphocytes

T R Hesketh, T Pozzan, G A Smith, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1983) 212 (3): 685–690.
https://doi.org/10.1042/bj2120685
Published: 15 June 1983
View article titled, Limits to the early increase in free cytoplasmic calcium concentration during the mitogenic stimulation of lymphocytes
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Articles

The bivalent-cation dependence of phosphatidylinositol synthesis in a cell-free system from lymphocytes

J P Moore, G A Smith, T R Hesketh, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1983) 212 (3): 691–697.
https://doi.org/10.1042/bj2120691
Published: 15 June 1983
View article titled, The bivalent-cation dependence of phosphatidylinositol synthesis in a cell-free system from lymphocytes
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Articles

Primary structure of bovine complement activation fragment C4a, the third anaphylatoxin. Purification and complete amino acid sequence

M A Smith, L M Gerrie, B Dunbar, J E Fothergill
Journal: Biochemical Journal
Biochem J (1982) 207 (2): 253–260.
https://doi.org/10.1042/bj2070253
Published: 01 November 1982
View article titled, Primary structure of bovine complement activation fragment C4a, the third anaphylatoxin. Purification and complete amino acid sequence
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Articles

The metabolism of l-tryptophan by liver cells prepared from adrenalectomized and streptozotocin-diabetic rats

S A Smith, C I Pogson
Journal: Biochemical Journal
Biochem J (1981) 200 (3): 605–609.
https://doi.org/10.1042/bj2000605
Published: 15 December 1981
View article titled, The metabolism of l-tryptophan by liver cells prepared from adrenalectomized and streptozotocin-diabetic rats
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Articles

The activities and intracellular distributions of enzymes of carbohydrate, lipid and ketone-body metabolism in lactating mammary glands from ruminants and non-ruminants

B Crabtree, D J Taylor, J E Coombs, R A Smith, S P Templer, G H Smith
Journal: Biochemical Journal
Biochem J (1981) 196 (3): 747–756.
https://doi.org/10.1042/bj1960747
Published: 15 June 1981
View article titled, The activities and intracellular distributions of enzymes of carbohydrate, lipid and ketone-body metabolism in lactating mammary glands from ruminants and non-ruminants
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Articles

Cholesterol in sarcoplasmic reticulum and the physiological significance of membrane fluidity

A Johannsson, C A Keightley, G A Smith, J C Metcalfe
Journal: Biochemical Journal
Biochem J (1981) 196 (2): 505–511.
https://doi.org/10.1042/bj1960505
Published: 15 May 1981
View article titled, Cholesterol in sarcoplasmic reticulum and the physiological significance of membrane fluidity
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